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10.21769/BioProtoc.4005 http://scihub22266oqcxt.onion/10.21769/BioProtoc.4005 34124305!8161125!34124305     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Bio+Protoc 2021 ; 11 (9): e4005 Nephropedia Template TP {{tp|p=34124305|t=2021. COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR |pdf=|usr=}}{{34124305}} gab.com Text COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR JO: Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34124305 #FOAvip The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume less reagents by increasing the capacity of testing and reducing the number of experiments performed, and are therefore especially suitable for under-developed countries with limited resources. Here, we propose a simple, reliable pooling strategy for COVID-19 testing using clinical nasopharyngeal (NP) and/or oropharyngeal (OP) swabs. The strategy includes the pooling of 10 NP/OP swabs for extraction and subsequent testing via quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR), and may also be applied to the screening of other pathogens. Twit Text FOAVip COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR ????Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34124305 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34124305 #FOAvip English Wikipedia <ref>{{Cite pmid|34124305}}</ref> COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR #MMPMID34124305 Voon K; Johari NA; Lim KL; Wong ST; Khaw LT; Wong SF; Chan EWL; Chan KK; Tan BK; Ramzi NH; Lim PKC; Sulaiman LH Bio Protoc 2021[May]; 11 (9): e4005 PMID34124305show ga The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume less reagents by increasing the capacity of testing and reducing the number of experiments performed, and are therefore especially suitable for under-developed countries with limited resources. Here, we propose a simple, reliable pooling strategy for COVID-19 testing using clinical nasopharyngeal (NP) and/or oropharyngeal (OP) swabs. The strategy includes the pooling of 10 NP/OP swabs for extraction and subsequent testing via quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR), and may also be applied to the screening of other pathogens. äCOVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time(epmc).pdf DeepDyve Pubget Overpricing