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10.1016/j.bios.2021.113418 http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113418 34119838!8182983!34119838     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34119838&cmd=llinks): Failed to open stream: HTTP request failed! 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A CRISPR-based and post-amplification coupled SARS-CoV-2 detection with a portable evanescent wave biosensor |pdf=|usr=}}{{34119838}} gab.com Text A CRISPR-based and post-amplification coupled SARS-CoV-2 detection with a portable evanescent wave biosensor JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=34119838 #FOAvip The continuing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, which causes coronavirus disease 2019 (COVID-19), has spread globally and its reliable diagnosis is one of the foremost priorities for protecting public health. Herein a rapid (<1 h), easy-to-implement, and accurate CRISPR-based evanescent wave fluorescence biosensing platform for detection of SARS-CoV-2 is reported. The collateral effect of Cas13a is combined with a universal autonomous enzyme-free hybridization chain reaction (HCR) by designing a cleavage hairpin reporter, which is cleaved upon target recognition, and hence releasing the initiator sequence to trigger the downstream HCR circuits. Detection of HCR assemblies is accomplished by first adsorbing to the desthiobiotin-modified optical fiber, followed by fluorescence emission induced by an evanescent field. Three Cas13a crRNAs targeting the genes of S, N and Orf1ab of SARS-CoV-2 are programmed to specifically target SARS-CoV-2 or broadly detect related coronavirus strains, such as MERS-CoV and SARS-CoV. The HCR amplification coupled Cas13a-based biosensing platform is capable of rapid detection of SARS-CoV-2 with attomolar sensitivity. This method is further validated by adding target RNA of SARS-CoV-2 in negative oropharyngeal swabs. The good discrimination capability of this technique demonstrates its promising potential for point-of-care diagnosis of COVID-19. Twit Text FOAVip A CRISPR-based and post-amplification coupled SARS-CoV-2 detection with a portable evanescent wave biosensor ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=34119838 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34119838 #FOAvip English Wikipedia <ref>{{Cite pmid|34119838}}</ref> A CRISPR-based and post-amplification coupled SARS-CoV-2 detection with a portable evanescent wave biosensor #MMPMID34119838 Yang Y; Liu J; Zhou X Biosens Bioelectron 2021[Oct]; 190 (ä): 113418 PMID34119838show ga The continuing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, which causes coronavirus disease 2019 (COVID-19), has spread globally and its reliable diagnosis is one of the foremost priorities for protecting public health. Herein a rapid (<1 h), easy-to-implement, and accurate CRISPR-based evanescent wave fluorescence biosensing platform for detection of SARS-CoV-2 is reported. The collateral effect of Cas13a is combined with a universal autonomous enzyme-free hybridization chain reaction (HCR) by designing a cleavage hairpin reporter, which is cleaved upon target recognition, and hence releasing the initiator sequence to trigger the downstream HCR circuits. Detection of HCR assemblies is accomplished by first adsorbing to the desthiobiotin-modified optical fiber, followed by fluorescence emission induced by an evanescent field. Three Cas13a crRNAs targeting the genes of S, N and Orf1ab of SARS-CoV-2 are programmed to specifically target SARS-CoV-2 or broadly detect related coronavirus strains, such as MERS-CoV and SARS-CoV. The HCR amplification coupled Cas13a-based biosensing platform is capable of rapid detection of SARS-CoV-2 with attomolar sensitivity. This method is further validated by adding target RNA of SARS-CoV-2 in negative oropharyngeal swabs. The good discrimination capability of this technique demonstrates its promising potential for point-of-care diagnosis of COVID-19. |*Biosensing Techniques[MESH] |*COVID-19[MESH] |CRISPR-Cas Systems[MESH] |Clustered Regularly Interspaced Short Palindromic Repeats[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques[MESH] |RNA, Viral[MESH] |SARS-CoV-2[MESH]A CRISPR-based and post-amplification coupled SARS-CoV-2 detection wit(epmc).pdf DeepDyve Pubget Overpricing