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10.1038/s41423-021-00717-5 http://scihub22266oqcxt.onion/10.1038/s41423-021-00717-5 34117370!8193015!34117370     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Mol+Immunol 2021 ; 18 (8): 1847-1860 Nephropedia Template TP {{tp|p=34117370|t=2021. Yeast display of MHC-II enables rapid identification of peptide ligands from protein antigens (RIPPA) |pdf=|usr=}}{{34117370}} gab.com Text Yeast display of MHC-II enables rapid identification of peptide ligands from protein antigens (RIPPA) JO: Cell Mol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=34117370 #FOAvip CD4(+) T cells orchestrate adaptive immune responses via binding of antigens to their receptors through specific peptide/MHC-II complexes. To study these responses, it is essential to identify protein-derived MHC-II peptide ligands that constitute epitopes for T cell recognition. However, generating cells expressing single MHC-II alleles and isolating these proteins for use in peptide elution or binding studies is time consuming. Here, we express human MHC alleles (HLA-DR4 and HLA-DQ6) as native, noncovalent alphabeta dimers on yeast cells for direct flow cytometry-based screening of peptide ligands from selected antigens. We demonstrate rapid, accurate identification of DQ6 ligands from pre-pro-hypocretin, a narcolepsy-related immunogenic target. We also identify 20 DR4-binding SARS-CoV-2 spike peptides homologous to SARS-CoV-1 epitopes, and one spike peptide overlapping with the reported SARS-CoV-2 epitope recognized by CD4(+) T cells from unexposed individuals carrying DR4 subtypes. Our method is optimized for immediate application upon the emergence of novel pathogens. Twit Text FOAVip Yeast display of MHC-II enables rapid identification of peptide ligands from protein antigens (RIPPA) ????Cell Mol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=34117370 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34117370 #FOAvip English Wikipedia <ref>{{Cite pmid|34117370}}</ref> Yeast display of MHC-II enables rapid identification of peptide ligands from protein antigens (RIPPA) #MMPMID34117370 Liu R; Jiang W; Mellins ED Cell Mol Immunol 2021[Aug]; 18 (8): 1847-1860 PMID34117370show ga CD4(+) T cells orchestrate adaptive immune responses via binding of antigens to their receptors through specific peptide/MHC-II complexes. To study these responses, it is essential to identify protein-derived MHC-II peptide ligands that constitute epitopes for T cell recognition. However, generating cells expressing single MHC-II alleles and isolating these proteins for use in peptide elution or binding studies is time consuming. Here, we express human MHC alleles (HLA-DR4 and HLA-DQ6) as native, noncovalent alphabeta dimers on yeast cells for direct flow cytometry-based screening of peptide ligands from selected antigens. We demonstrate rapid, accurate identification of DQ6 ligands from pre-pro-hypocretin, a narcolepsy-related immunogenic target. We also identify 20 DR4-binding SARS-CoV-2 spike peptides homologous to SARS-CoV-1 epitopes, and one spike peptide overlapping with the reported SARS-CoV-2 epitope recognized by CD4(+) T cells from unexposed individuals carrying DR4 subtypes. Our method is optimized for immediate application upon the emergence of novel pathogens. |*Two-Hybrid System Techniques[MESH] |CD4-Positive T-Lymphocytes/immunology/*metabolism/virology[MESH] |COVID-19/genetics/immunology/*metabolism[MESH] |Epitopes, T-Lymphocyte/genetics/immunology/*metabolism[MESH] |Flow Cytometry[MESH] |HLA-DQ Antigens/genetics/immunology/*metabolism[MESH] |HLA-DR4 Antigen/genetics/immunology/*metabolism[MESH] |Ligands[MESH] |Saccharomyces cerevisiae/genetics/immunology/*metabolism[MESH]Yeast display of MHC-II enables rapid identification of peptide ligand(epmc).pdf DeepDyve Pubget Overpricing