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10.1016/j.diagmicrobio.2021.115425 http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2021.115425 34116343!8116316!34116343     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Diagn+Microbiol+Infect+Dis 2021 ; 101 (1): 115425 Nephropedia Template TP {{tp|p=34116343|t=2021. Detection of SARS-CoV-2 IgG antibodies in dried blood spots |pdf=|usr=}}{{34116343}} gab.com Text Detection of SARS-CoV-2 IgG antibodies in dried blood spots JO: Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34116343 #FOAvip The COVID-19 pandemic led to development of numerous serologic tests. To obviate the need for phlebotomy services, we validated dried blood spots (DBS) for anti-SARS-CoV-2 serologic testing. Immunoglobulins were extracted from 3 mm DBS punches and the extracts were analyzed using the Euroimmun anti-SARS-CoV-2 IgG ELISA. Various pre-analytical factors were studied. Results were favorable for DBS stored for at least 67 days at or below 37 degrees C. Comparison between paired serum and DBS specimens tested by the Euroimmun ELISA showed 96.8% and 81.3% positive and negative agreement, respectively, indicating that confirmatory testing of positive Euroimmun results on DBS extracts is necessary to achieve clinical accuracy. Our findings suggest that any SARS-CoV-2 antibody assay that requires pre-dilution of serum is amenable to DBS as an alternate specimen type that is relatively low cost, self-collectable, stable, can be shipped by standard mail and could be used to establish the seroprevalence of large populations. Twit Text FOAVip Detection of SARS-CoV-2 IgG antibodies in dried blood spots ????Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34116343 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34116343 #FOAvip English Wikipedia <ref>{{Cite pmid|34116343}}</ref> Detection of SARS-CoV-2 IgG antibodies in dried blood spots #MMPMID34116343 Turgeon CT; Sanders KA; Granger D; Nett SL; Hilgart H; Matern D; Theel ES Diagn Microbiol Infect Dis 2021[Sep]; 101 (1): 115425 PMID34116343show ga The COVID-19 pandemic led to development of numerous serologic tests. To obviate the need for phlebotomy services, we validated dried blood spots (DBS) for anti-SARS-CoV-2 serologic testing. Immunoglobulins were extracted from 3 mm DBS punches and the extracts were analyzed using the Euroimmun anti-SARS-CoV-2 IgG ELISA. Various pre-analytical factors were studied. Results were favorable for DBS stored for at least 67 days at or below 37 degrees C. Comparison between paired serum and DBS specimens tested by the Euroimmun ELISA showed 96.8% and 81.3% positive and negative agreement, respectively, indicating that confirmatory testing of positive Euroimmun results on DBS extracts is necessary to achieve clinical accuracy. Our findings suggest that any SARS-CoV-2 antibody assay that requires pre-dilution of serum is amenable to DBS as an alternate specimen type that is relatively low cost, self-collectable, stable, can be shipped by standard mail and could be used to establish the seroprevalence of large populations. |*COVID-19 Serological Testing/instrumentation[MESH] |*Dried Blood Spot Testing/instrumentation[MESH] |Antibodies, Viral/*blood[MESH] |COVID-19/*diagnosis[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Humans[MESH] |Immunoglobulin G/*blood[MESH] |SARS-CoV-2/immunology/*isolation & purification[MESH] |Sensitivity and Specificity[MESH]Detection of SARS-CoV-2 IgG antibodies in dried blood spots (epmc).pdf DeepDyve Pubget Overpricing