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10.1038/s41598-021-91300-5 http://scihub22266oqcxt.onion/10.1038/s41598-021-91300-5 34112850!8192771!34112850     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34112850&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 12330 Nephropedia Template TP {{tp|p=34112850|t=2021. High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay |pdf=|usr=}}{{34112850}} gab.com Text High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34112850 #FOAvip SARS-CoV-2 emerged in late 2019 and has since spread around the world, causing a pandemic of the respiratory disease COVID-19. Detecting antibodies against the virus is an essential tool for tracking infections and developing vaccines. Such tests, primarily utilizing the enzyme-linked immunosorbent assay (ELISA) principle, can be either qualitative (reporting positive/negative results) or quantitative (reporting a value representing the quantity of specific antibodies). Quantitation is vital for determining stability or decline of antibody titers in convalescence, efficacy of different vaccination regimens, and detection of asymptomatic infections. Quantitation typically requires two-step ELISA testing, in which samples are first screened in a qualitative assay and positive samples are subsequently analyzed as a dilution series. To overcome the throughput limitations of this approach, we developed a simpler and faster system that is highly automatable and achieves quantitation in a single-dilution screening format with sensitivity and specificity comparable to those of ELISA. Twit Text FOAVip High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34112850 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34112850 #FOAvip English Wikipedia <ref>{{Cite pmid|34112850}}</ref> High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay #MMPMID34112850 Kainulainen MH; Bergeron E; Chatterjee P; Chapman AP; Lee J; Chida A; Tang X; Wharton RE; Mercer KB; Petway M; Jenks HM; Flietstra TD; Schuh AJ; Satheshkumar PS; Chaitram JM; Owen SM; McMullan LK; Flint M; Finn MG; Goldstein JM; Montgomery JM; Spiropoulou CF Sci Rep 2021[Jun]; 11 (1): 12330 PMID34112850show ga SARS-CoV-2 emerged in late 2019 and has since spread around the world, causing a pandemic of the respiratory disease COVID-19. Detecting antibodies against the virus is an essential tool for tracking infections and developing vaccines. Such tests, primarily utilizing the enzyme-linked immunosorbent assay (ELISA) principle, can be either qualitative (reporting positive/negative results) or quantitative (reporting a value representing the quantity of specific antibodies). Quantitation is vital for determining stability or decline of antibody titers in convalescence, efficacy of different vaccination regimens, and detection of asymptomatic infections. Quantitation typically requires two-step ELISA testing, in which samples are first screened in a qualitative assay and positive samples are subsequently analyzed as a dilution series. To overcome the throughput limitations of this approach, we developed a simpler and faster system that is highly automatable and achieves quantitation in a single-dilution screening format with sensitivity and specificity comparable to those of ELISA. |Animals[MESH] |Antibodies, Viral/*blood/immunology[MESH] |COVID-19 Serological Testing/economics/methods[MESH] |COVID-19/*blood/diagnosis/immunology[MESH] |Enzyme-Linked Immunosorbent Assay/economics/methods[MESH] |Humans[MESH] |Immunoglobulin G/blood/immunology[MESH] |Immunoglobulin M/blood/immunology[MESH] |Mice[MESH]High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilu(epmc).pdf DeepDyve Pubget Overpricing