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  • Comparison of renal histopathology and gene expression profiles between severe COVID-19 and bacterial sepsis in critically ill patients #MMPMID34112226
  • Volbeda M; Jou-Valencia D; van den Heuvel MC; Knoester M; Zwiers PJ; Pillay J; Berger SP; van der Voort PHJ; Zijlstra JG; van Meurs M; Moser J
  • Crit Care 2021[Jun]; 25 (1): 202 PMID34112226show ga
  • BACKGROUND: The mechanisms driving acute kidney injury (AKI) in critically ill COVID-19 patients are unclear. We collected kidney biopsies from COVID-19 AKI patients within 30 min after death in order to examine the histopathology and perform mRNA expression analysis of genes associated with renal injury. METHODS: This study involved histopathology and mRNA analyses of postmortem kidney biopsies collected from patients with COVID-19 (n = 6) and bacterial sepsis (n = 27). Normal control renal tissue was obtained from patients undergoing total nephrectomy (n = 12). The mean length of ICU admission-to-biopsy was 30 days for COVID-19 and 3-4 days for bacterial sepsis patients. RESULTS: We did not detect SARS-CoV-2 RNA in kidney biopsies from COVID-19-AKI patients yet lung tissue from the same patients was PCR positive. Extensive acute tubular necrosis (ATN) and peritubular thrombi were distinct histopathology features of COVID-19-AKI compared to bacterial sepsis-AKI. ACE2 mRNA levels in both COVID-19 (fold change 0.42, p = 0.0002) and bacterial sepsis patients (fold change 0.24, p < 0.0001) were low compared to control. The mRNA levels of injury markers NGAL and KIM-1 were unaltered compared to control tissue but increased in sepsis-AKI patients. Markers for inflammation and endothelial activation were unaltered in COVID-19 suggesting a lack of renal inflammation. Renal mRNA levels of endothelial integrity markers CD31, PV-1 and VE-Cadherin did not differ from control individuals yet were increased in bacterial sepsis patients (CD31 fold change 2.3, p = 0.0006, PV-1 fold change 1.5, p = 0.008). Angiopoietin-1 mRNA levels were downregulated in renal tissue from both COVID-19 (fold change 0.27, p < 0.0001) and bacterial sepsis patients (fold change 0.67, p < 0.0001) compared to controls. Moreover, low Tie2 mRNA expression (fold change 0.33, p = 0.037) and a disturbed VEGFR2/VEGFR3 ratio (fold change 0.09, p < 0.0001) suggest decreased microvascular flow in COVID-19. CONCLUSIONS: In a small cohort of postmortem kidney biopsies from COVID-19 patients, we observed distinct histopathological and gene expression profiles between COVID-19-AKI and bacterial sepsis-AKI. COVID-19 was associated with more severe ATN and microvascular thrombosis coupled with decreased microvascular flow, yet minimal inflammation. Further studies are required to determine whether these observations are a result of true pathophysiological differences or related to the timing of biopsy after disease onset.
  • |Acute Kidney Injury/etiology/physiopathology[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Analysis of Variance[MESH]
  • |COVID-19/genetics/*pathology/physiopathology[MESH]
  • |Critical Illness/therapy[MESH]
  • |Female[MESH]
  • |Gene Expression/*genetics[MESH]
  • |Humans[MESH]
  • |Intensive Care Units/organization & administration/statistics & numerical data[MESH]
  • |Kidney/*pathology/*physiopathology[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Sepsis/genetics/*pathology/physiopathology[MESH]
  • |Simplified Acute Physiology Score[MESH]

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  • suck abstract from ncbi

    202 1.25 2021