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10.1016/j.diagmicrobio.2021.115424

http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2021.115424
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34111651!ä!34111651

suck abstract from ncbi


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pmid34111651      Diagn+Microbiol+Infect+Dis 2021 ; 101 (1): 115424
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  • Usefulness of the GenMark ePlex RPP assay for the detection of respiratory viruses compared to the FTD21 multiplex RT-PCR #MMPMID34111651
  • Steiner F; Schmutz S; Gosert R; Huder JB; Redli PM; Capaul R; Hirsch HH; Boni J; Zbinden A
  • Diagn Microbiol Infect Dis 2021[Sep]; 101 (1): 115424 PMID34111651show ga
  • Cartridge-based multiplex panels covering numerous pathogens offer an advantage of minimal hands-on-time and short time to result to commercial RT-PCR assays. In this study, we evaluated the performance of the ePlex respiratory pathogen panel (RPP) compared to the Fast Track Diagnostics Respiratory pathogens 21 multiplex RT-PCR assay (FTD21) using 400 clinical respiratory samples. Discrepant results were further analysed by a reference nucleic acid amplification testing (NAT) and a composite reference approach was used for final interpretation. Discordant results were observed in 56 targets corresponding to 54 samples. Sensitivities and specificities were 85.5% and 99.9% for the ePlex RPP and 95.8% and 99.7% for the FTD21 system, respectively. Altogether, the ePlex RPP is a valuable tool for the rapid detection of a number of different respiratory viruses with the exception of the coronavirus family (low sensitivity ranging from 50-80%) and samples with a low pathogen load (Ct values >33).
  • |*Molecular Diagnostic Techniques[MESH]
  • |*Multiplex Polymerase Chain Reaction[MESH]
  • |Diagnostic Tests, Routine[MESH]
  • |Humans[MESH]
  • |Respiratory Tract Infections/*diagnosis/virology[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Time Factors[MESH]


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