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10.1371/journal.pone.0252964

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34111203!8191987!34111203
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suck abstract from ncbi


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pmid34111203      PLoS+One 2021 ; 16 (6): e0252964
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  • Detection of SARS-CoV-2 using qRT-PCR in saliva obtained from asymptomatic or mild COVID-19 patients, comparative analysis with matched nasopharyngeal samples #MMPMID34111203
  • Ota K; Yanagihara K; Sasaki D; Kaku N; Uno N; Sakamoto K; Kosai K; Miyazaki T; Hasegawa H; Fujita A; Tashiro M; Tanaka T; Izumikawa K; Ariyoshi K; Mukae H; Yasuda J; Morita K; Kohno S
  • PLoS One 2021[]; 16 (6): e0252964 PMID34111203show ga
  • OBJECTIVES: The accurate detection of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is essential for the diagnosis of coronavirus disease 2019 (COVID-19). We compared the quantitative RT-PCR results between nasopharyngeal swabs and saliva specimens. METHODS: A COVID-19 outbreak occurred on a cruise ship at Nagasaki port, Japan. We obtained 123 nasopharyngeal swabs and saliva each from asymptomatic or mild patients in the late phase of infection. RESULTS: The intervals from the diagnosis to the sampling were 25.5 days for nasopharyngeal swabs and 28.9 days for saliva. The positive rate was 19.5% (24/123) for nasopharyngeal swabs and 38.2% (47/123) for saliva (P = 0.48). The quantified viral copies (mean +/- SEM copies/5 mul) were 9.3+/-2.6 in nasopharyngeal swabs and 920+/-850 in saliva (P = 0.0006). CONCLUSIONS: The advantages of saliva specimens include positive rate improvement and accurate viral load detection. Saliva may be used as a reliable sample for SARS-CoV-2 detection.
  • |*COVID-19 Nucleic Acid Testing[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Humans[MESH]
  • |Nasopharynx/*virology[MESH]
  • |SARS-CoV-2/*isolation & purification[MESH]
  • |Saliva/*virology[MESH]


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