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10.3343/alm.2021.41.6.568 http://scihub22266oqcxt.onion/10.3343/alm.2021.41.6.568 34108284!8203440!34108284     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Ann+Lab+Med 2021 ; 41 (6): 568-576 Nephropedia Template TP {{tp|p=34108284|t=2021. Combination of a SARS-CoV-2 IgG Assay and RT-PCR for Improved COVID-19 Diagnosis |pdf=|usr=}}{{34108284}} gab.com Text Combination of a SARS-CoV-2 IgG Assay and RT-PCR for Improved COVID-19 Diagnosis JO: Ann Lab Med http://www.kidney.de/pget.php?&tw=1&pmid=34108284 #FOAvip BACKGROUND: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is generally diagnosed by reverse transcription (RT)-PCR or serological assays. The SARS-CoV-2 viral load decreases a few days after symptom onset. Thus, the RT-PCR sensitivity peaks at three days after symptom onset (approximately 80%). We evaluated the performance of the ARCHITECT((R)) SARS-CoV-2 IgG assay (henceforth termed IgG assay; Abbott Laboratories, Lake County, IL, USA), and the combination of RT-PCR and the IgG assay for COVID-19 diagnosis. METHODS: In this retrospective study, 206 samples from 70 COVID-19 cases at two hospitals in Tokyo that were positive using RT-PCR were used to analyze the diagnostic sensitivity. RT-PCR-negative (N=166), COVID-19-unrelated (N=418), and Japanese Red Cross Society (N=100) samples were used to evaluate specificity. RESULTS: Sensitivity increased daily after symptom onset and exceeded 84.4% after 10 days. Specificity ranged from 98.2% to 100% for samples from the three case groups. Seroconversion was confirmed from 9 to 20 days after symptom onset in 18 out of 32 COVID-19 cases with multiple samples and from another case with a positive result in the IgG assay for the first available sample. CONCLUSIONS: The combination of RT-PCR and IgG assay improves the robustness of laboratory diagnostics by compensating for the limitations of each method. Twit Text FOAVip Combination of a SARS-CoV-2 IgG Assay and RT-PCR for Improved COVID-19 Diagnosis ????Ann Lab Med http://www.kidney.de/pget.php?&tw=1&pmid=34108284 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34108284 #FOAvip English Wikipedia <ref>{{Cite pmid|34108284}}</ref> Combination of a SARS-CoV-2 IgG Assay and RT-PCR for Improved COVID-19 Diagnosis #MMPMID34108284 Aoki K; Takai K; Nagasawa T; Kashiwagi K; Mori N; Matsubayashi K; Satake M; Tanaka I; Kodama N; Shimodaira T; Ishii Y; Miyazaki T; Ishii T; Morita T; Yoshimura T; Tateda K Ann Lab Med 2021[Nov]; 41 (6): 568-576 PMID34108284show ga BACKGROUND: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is generally diagnosed by reverse transcription (RT)-PCR or serological assays. The SARS-CoV-2 viral load decreases a few days after symptom onset. Thus, the RT-PCR sensitivity peaks at three days after symptom onset (approximately 80%). We evaluated the performance of the ARCHITECT((R)) SARS-CoV-2 IgG assay (henceforth termed IgG assay; Abbott Laboratories, Lake County, IL, USA), and the combination of RT-PCR and the IgG assay for COVID-19 diagnosis. METHODS: In this retrospective study, 206 samples from 70 COVID-19 cases at two hospitals in Tokyo that were positive using RT-PCR were used to analyze the diagnostic sensitivity. RT-PCR-negative (N=166), COVID-19-unrelated (N=418), and Japanese Red Cross Society (N=100) samples were used to evaluate specificity. RESULTS: Sensitivity increased daily after symptom onset and exceeded 84.4% after 10 days. Specificity ranged from 98.2% to 100% for samples from the three case groups. Seroconversion was confirmed from 9 to 20 days after symptom onset in 18 out of 32 COVID-19 cases with multiple samples and from another case with a positive result in the IgG assay for the first available sample. CONCLUSIONS: The combination of RT-PCR and IgG assay improves the robustness of laboratory diagnostics by compensating for the limitations of each method. |Antibodies, Viral/analysis[MESH] |COVID-19 Testing[MESH] |COVID-19/*diagnosis/virology[MESH] |Humans[MESH] |Immunoglobulin G/*analysis[MESH] |Longitudinal Studies[MESH] |RNA, Viral/*analysis/metabolism[MESH] |Reagent Kits, Diagnostic[MESH] |Retrospective Studies[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |SARS-CoV-2/genetics/immunology/isolation & purification[MESH]Combination of a SARS-CoV-2 IgG Assay and RT-PCR for Improved COVID-19(epmc).pdf DeepDyve Pubget Overpricing