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10.1038/s41467-021-23779-5 http://scihub22266oqcxt.onion/10.1038/s41467-021-23779-5 34103499!8187723!34103499     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 3431 Nephropedia Template TP {{tp|p=34103499|t=2021. A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses |pdf=|usr=}}{{34103499}} gab.com Text A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34103499 #FOAvip The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We demonstrate that despite the large size of the viral RNA genome (~30 kb), infectious full-length cDNA is readily assembled in vitro by a circular polymerase extension reaction (CPER) methodology without the need for technically demanding intermediate steps. Overlapping cDNA fragments are generated from viral RNA and assembled together with a linker fragment containing CMV promoter into a circular full-length viral cDNA in a single reaction. Transfection of the circular cDNA into mammalian cells results in the recovery of infectious SARS-CoV-2 virus that exhibits properties comparable to the parental virus in vitro and in vivo. CPER is also used to generate insect-specific Casuarina virus with ~20 kb genome and the human pathogens Ross River virus (Alphavirus) and Norovirus (Calicivirus), with the latter from a clinical sample. Additionally, reporter and mutant viruses are generated and employed to study virus replication and virus-receptor interactions. Twit Text FOAVip A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34103499 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34103499 #FOAvip English Wikipedia <ref>{{Cite pmid|34103499}}</ref> A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses #MMPMID34103499 Amarilla AA; Sng JDJ; Parry R; Deerain JM; Potter JR; Setoh YX; Rawle DJ; Le TT; Modhiran N; Wang X; Peng NYG; Torres FJ; Pyke A; Harrison JJ; Freney ME; Liang B; McMillan CLD; Cheung STM; Guevara DJDC; Hardy JM; Bettington M; Muller DA; Coulibaly F; Moore F; Hall RA; Young PR; Mackenzie JM; Hobson-Peters J; Suhrbier A; Watterson D; Khromykh AA Nat Commun 2021[Jun]; 12 (1): 3431 PMID34103499show ga The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We demonstrate that despite the large size of the viral RNA genome (~30 kb), infectious full-length cDNA is readily assembled in vitro by a circular polymerase extension reaction (CPER) methodology without the need for technically demanding intermediate steps. Overlapping cDNA fragments are generated from viral RNA and assembled together with a linker fragment containing CMV promoter into a circular full-length viral cDNA in a single reaction. Transfection of the circular cDNA into mammalian cells results in the recovery of infectious SARS-CoV-2 virus that exhibits properties comparable to the parental virus in vitro and in vivo. CPER is also used to generate insect-specific Casuarina virus with ~20 kb genome and the human pathogens Ross River virus (Alphavirus) and Norovirus (Calicivirus), with the latter from a clinical sample. Additionally, reporter and mutant viruses are generated and employed to study virus replication and virus-receptor interactions. |*Reverse Genetics[MESH] |Amino Acid Sequence[MESH] |Animals[MESH] |Base Sequence[MESH] |Chlorocebus aethiops[MESH] |Culicidae/virology[MESH] |Furin/metabolism[MESH] |Genome, Viral[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Mice[MESH] |Mutation/genetics[MESH] |NIH 3T3 Cells[MESH] |Polymerase Chain Reaction[MESH] |RAW 264.7 Cells[MESH] |Receptors, Virus/metabolism[MESH] |SARS-CoV-2/*genetics[MESH] |Vero Cells[MESH] |Viral Proteins/chemistry[MESH]A versatile reverse genetics platform for SARS-CoV-2 and other positiv(epmc).pdf DeepDyve Pubget Overpricing