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10.1039/d1lc00293g http://scihub22266oqcxt.onion/10.1039/d1lc00293g 34100058!8277744!34100058     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Lab+Chip 2021 ; 21 (14): 2730-2737 Nephropedia Template TP {{tp|p=34100058|t=2021. Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2 |pdf=|usr=}}{{34100058}} gab.com Text Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2 JO: Lab Chip http://www.kidney.de/pget.php?&tw=1&pmid=34100058 #FOAvip The COVID-19 pandemic, caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), has become a public health emergency and widely spread around the world. Rapid, accurate and early diagnosis of COVID-19 infection plays a crucial role in breaking this pandemic. However, the detection accuracy is limited for current single-gene diagnosis of SARS-CoV-2. Herein, we develop an autonomous lab-on-paper platform for multiplex gene diagnosis of SARS-CoV-2 by combining reverse transcription recombinase polymerase amplification (RT-RPA) and CRISPR-Cas12a detection. The autonomous lab-on-paper is capable of simultaneously detecting nucleoprotein (N) gene and spike (S) gene of SARS-CoV-2 virus as well as human housekeeping RNAse P gene (an internal control) in a single clinical sample. With the developed platform, 102 copies viral RNA per test can be detected within one hour. Also, the lab-on-paper platform has been used to detect 21 swab clinical samples and obtains a comparable performance to the conventional RT-PCR method. Thus, the developed lab-on-paper platform holds great potential for rapid, sensitive, reliable, multiple molecular diagnostics of COVID-19 and other infectious diseases in resource-limited settings. Twit Text FOAVip Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2 ????Lab Chip http://www.kidney.de/pget.php?&tw=1&pmid=34100058 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34100058 #FOAvip English Wikipedia <ref>{{Cite pmid|34100058}}</ref> Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2 #MMPMID34100058 Yin K; Ding X; Li Z; Sfeir MM; Ballesteros E; Liu C Lab Chip 2021[Jul]; 21 (14): 2730-2737 PMID34100058show ga The COVID-19 pandemic, caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), has become a public health emergency and widely spread around the world. Rapid, accurate and early diagnosis of COVID-19 infection plays a crucial role in breaking this pandemic. However, the detection accuracy is limited for current single-gene diagnosis of SARS-CoV-2. Herein, we develop an autonomous lab-on-paper platform for multiplex gene diagnosis of SARS-CoV-2 by combining reverse transcription recombinase polymerase amplification (RT-RPA) and CRISPR-Cas12a detection. The autonomous lab-on-paper is capable of simultaneously detecting nucleoprotein (N) gene and spike (S) gene of SARS-CoV-2 virus as well as human housekeeping RNAse P gene (an internal control) in a single clinical sample. With the developed platform, 102 copies viral RNA per test can be detected within one hour. Also, the lab-on-paper platform has been used to detect 21 swab clinical samples and obtains a comparable performance to the conventional RT-PCR method. Thus, the developed lab-on-paper platform holds great potential for rapid, sensitive, reliable, multiple molecular diagnostics of COVID-19 and other infectious diseases in resource-limited settings. |*COVID-19[MESH] |*Pandemics[MESH] |CRISPR-Cas Systems/genetics[MESH] |Clustered Regularly Interspaced Short Palindromic Repeats[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2[MESH]Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of S(epmc).pdf DeepDyve Pubget Overpricing