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10.1038/s41598-021-91457-z http://scihub22266oqcxt.onion/10.1038/s41598-021-91457-z 34099796!8185005!34099796     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 11899 Nephropedia Template TP {{tp|p=34099796|t=2021. Complementary methods for SARS-CoV-2 diagnosis in times of material shortage |pdf=|usr=}}{{34099796}} gab.com Text Complementary methods for SARS-CoV-2 diagnosis in times of material shortage JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34099796 #FOAvip The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance of self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab and rayon swab), of reagents for classical RNA extraction (phenol/guanidine-based protocol against a commercial kit), and of intercalating dye-based one-step quantitative reverse transcription real-time PCRs (RT-qPCR) compared against the gold standard hydrolysis probe-based assays for SARS-CoV-2 detection. The study found sampling with Q-tips, RNA extraction with classical protocol and intercalating dye-based RT-qPCR as a reliable and comparably sensitive strategy for detection of SARS-CoV-2-particularly valuable in the current period with a resurgent and dramatic increase in SARS-CoV-2 infections and growing shortage of diagnostic materials especially for regions limited in resources. Twit Text FOAVip Complementary methods for SARS-CoV-2 diagnosis in times of material shortage ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34099796 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34099796 #FOAvip English Wikipedia <ref>{{Cite pmid|34099796}}</ref> Complementary methods for SARS-CoV-2 diagnosis in times of material shortage #MMPMID34099796 Sandri TL; Inoue J; Geiger J; Griesbaum JM; Heinzel C; Burnet M; Fendel R; Kremsner PG; Held J; Kreidenweiss A Sci Rep 2021[Jun]; 11 (1): 11899 PMID34099796show ga The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance of self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab and rayon swab), of reagents for classical RNA extraction (phenol/guanidine-based protocol against a commercial kit), and of intercalating dye-based one-step quantitative reverse transcription real-time PCRs (RT-qPCR) compared against the gold standard hydrolysis probe-based assays for SARS-CoV-2 detection. The study found sampling with Q-tips, RNA extraction with classical protocol and intercalating dye-based RT-qPCR as a reliable and comparably sensitive strategy for detection of SARS-CoV-2-particularly valuable in the current period with a resurgent and dramatic increase in SARS-CoV-2 infections and growing shortage of diagnostic materials especially for regions limited in resources. |*COVID-19 Testing/methods[MESH] |*Specimen Handling/methods[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH] |RNA, Viral/*genetics[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH] |Reverse Transcription/physiology[MESH] |SARS-CoV-2/*pathogenicity[MESH]Complementary methods for SARS-CoV-2 diagnosis in times of material sh(epmc).pdf DeepDyve Pubget Overpricing