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10.1016/bs.mie.2020.12.020 http://scihub22266oqcxt.onion/10.1016/bs.mie.2020.12.020 34099172!7839409!34099172     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Enzymol 2021 ; 653 (ä): 207-235 Nephropedia Template TP {{tp|p=34099172|t=2021. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs |pdf=|usr=}}{{34099172}} gab.com Text SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs JO: Methods Enzymol http://www.kidney.de/pget.php?&tw=1&pmid=34099172 #FOAvip The SARS-CoV-2 3a protein is a putative ion channel implicated in virus life cycle and pathogenesis. We recently expressed, purified, and reconstituted 3a into lipid nanodiscs to solve its structure by cryo-EM to 2.1A resolution. In this chapter, we describe methods we developed in order to facilitate the study of this protein in other laboratories. We emphasize factors that enabled rapid progression from gene sequence to reconstituted protein (3 weeks in the case of 3a) and provide general observations and tips for adapting these protocols to other membrane proteins of interest. Twit Text FOAVip SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs ????Methods Enzymol http://www.kidney.de/pget.php?&tw=1&pmid=34099172 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34099172 #FOAvip English Wikipedia <ref>{{Cite pmid|34099172}}</ref> SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs #MMPMID34099172 Kern DM; Brohawn SG Methods Enzymol 2021[]; 653 (ä): 207-235 PMID34099172show ga The SARS-CoV-2 3a protein is a putative ion channel implicated in virus life cycle and pathogenesis. We recently expressed, purified, and reconstituted 3a into lipid nanodiscs to solve its structure by cryo-EM to 2.1A resolution. In this chapter, we describe methods we developed in order to facilitate the study of this protein in other laboratories. We emphasize factors that enabled rapid progression from gene sequence to reconstituted protein (3 weeks in the case of 3a) and provide general observations and tips for adapting these protocols to other membrane proteins of interest. |*Nanostructures[MESH] |Ion Channels/*chemistry[MESH] |Lipid Bilayers/chemistry[MESH] |SARS-CoV-2/*chemistry[MESH] |Viral Envelope Proteins/*chemistry[MESH]SARS-CoV-2 3a expression, purification, and reconstitution into lipid (epmc).pdf DeepDyve Pubget Overpricing