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10.1186/s12929-021-00740-8 http://scihub22266oqcxt.onion/10.1186/s12929-021-00740-8 34098950!8182997!34098950     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biomed+Sci 2021 ; 28 (1): 43 Nephropedia Template TP {{tp|p=34098950|t=2021. Identification of COVID-19 B-cell epitopes with phage-displayed peptide library |pdf=|usr=}}{{34098950}} gab.com Text Identification of COVID-19 B-cell epitopes with phage-displayed peptide library JO: J Biomed Sci http://www.kidney.de/pget.php?&tw=1&pmid=34098950 #FOAvip BACKGROUND: Coronavirus disease 19 (COVID-19) first appeared in the city of Wuhan, in the Hubei province of China. Since its emergence, the COVID-19-causing virus, SARS-CoV-2, has been rapidly transmitted around the globe, overwhelming the medical care systems in many countries and leading to more than 3.3 million deaths. Identification of immunological epitopes on the virus would be highly useful for the development of diagnostic tools and vaccines that will be critical to limiting further spread of COVID-19. METHODS: To find disease-specific B-cell epitopes that correspond to or mimic natural epitopes, we used phage display technology to determine the targets of specific antibodies present in the sera of immune-responsive COVID-19 patients. Enzyme-linked immunosorbent assays were further applied to assess competitive antibody binding and serological detection. VaxiJen, BepiPred-2.0 and DiscoTope 2.0 were utilized for B-cell epitope prediction. PyMOL was used for protein structural analysis. RESULTS: 36 enriched peptides were identified by biopanning with antibodies from two COVID-19 patients; the peptides 4 motifs with consensus residues corresponding to two potential B-cell epitopes on SARS-CoV-2 viral proteins. The putative epitopes and hit peptides were then synthesized for validation by competitive antibody binding and serological detection. CONCLUSIONS: The identified B-cell epitopes on SARS-CoV-2 may aid investigations into COVID-19 pathogenesis and facilitate the development of epitope-based serological diagnostics and vaccines. Twit Text FOAVip Identification of COVID-19 B-cell epitopes with phage-displayed peptide library ????J Biomed Sci http://www.kidney.de/pget.php?&tw=1&pmid=34098950 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34098950 #FOAvip English Wikipedia <ref>{{Cite pmid|34098950}}</ref> Identification of COVID-19 B-cell epitopes with phage-displayed peptide library #MMPMID34098950 Guo JY; Liu IJ; Lin HT; Wang MJ; Chang YL; Lin SC; Liao MY; Hsu WC; Lin YL; Liao JC; Wu HC J Biomed Sci 2021[Jun]; 28 (1): 43 PMID34098950show ga BACKGROUND: Coronavirus disease 19 (COVID-19) first appeared in the city of Wuhan, in the Hubei province of China. Since its emergence, the COVID-19-causing virus, SARS-CoV-2, has been rapidly transmitted around the globe, overwhelming the medical care systems in many countries and leading to more than 3.3 million deaths. Identification of immunological epitopes on the virus would be highly useful for the development of diagnostic tools and vaccines that will be critical to limiting further spread of COVID-19. METHODS: To find disease-specific B-cell epitopes that correspond to or mimic natural epitopes, we used phage display technology to determine the targets of specific antibodies present in the sera of immune-responsive COVID-19 patients. Enzyme-linked immunosorbent assays were further applied to assess competitive antibody binding and serological detection. VaxiJen, BepiPred-2.0 and DiscoTope 2.0 were utilized for B-cell epitope prediction. PyMOL was used for protein structural analysis. RESULTS: 36 enriched peptides were identified by biopanning with antibodies from two COVID-19 patients; the peptides 4 motifs with consensus residues corresponding to two potential B-cell epitopes on SARS-CoV-2 viral proteins. The putative epitopes and hit peptides were then synthesized for validation by competitive antibody binding and serological detection. CONCLUSIONS: The identified B-cell epitopes on SARS-CoV-2 may aid investigations into COVID-19 pathogenesis and facilitate the development of epitope-based serological diagnostics and vaccines. |*COVID-19/genetics/immunology[MESH] |*Epitopes, B-Lymphocyte/genetics/immunology[MESH] |*Peptide Library[MESH] |*SARS-CoV-2/genetics/immunology[MESH] |*Viral Proteins/genetics/immunology[MESH]Identification of COVID-19 B-cell epitopes with phage-displayed peptid(epmc).pdf DeepDyve Pubget Overpricing