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10.1016/j.bios.2021.113388 http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113388 34098362!8166042!34098362     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 190 (ä): 113388 Nephropedia Template TP {{tp|p=34098362|t=2021. A single snapshot multiplex immunoassay platform utilizing dense test lines based on engineered beads |pdf=|usr=}}{{34098362}} gab.com Text A single snapshot multiplex immunoassay platform utilizing dense test lines based on engineered beads JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=34098362 #FOAvip Co-circulation of coronavirus disease 2019 (COVID-19) and dengue fever has been reported. Accurate and timely multiplex diagnosis is required to prevent future pandemics. Here, we developed an innovative microfluidic chip that enables a snapshot multiplex immunoassay for timely on-site response and offers unprecedented multiplexing capability with an operating procedure similar to that of lateral flow assays. An open microchannel assembly of individually engineered microbeads was developed to construct nine high-density test lines, which can be imaged in a 1 mm(2) field-of-view. Thus, simultaneous detection of multiple antibodies would be achievable in a single high-resolution snapshot. Next, we developed a novel pixel intensity-based imaging process to distinguish effective and non-specific fluorescence signals, thereby improving the reliability of this fluorescence-based immunoassay. Finally, the chip specifically identified and classified random combinations of arbovirus (Zika, dengue, and chikungunya viruses) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies within 30 min. Therefore, we believe that this snapshot multiplex immunoassay chip is a powerful diagnostic tool to control current and future pandemics. Twit Text FOAVip A single snapshot multiplex immunoassay platform utilizing dense test lines based on engineered beads ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=34098362 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34098362 #FOAvip English Wikipedia <ref>{{Cite pmid|34098362}}</ref> A single snapshot multiplex immunoassay platform utilizing dense test lines based on engineered beads #MMPMID34098362 Lee W; Kim H; Bae PK; Lee S; Yang S; Kim J Biosens Bioelectron 2021[Oct]; 190 (ä): 113388 PMID34098362show ga Co-circulation of coronavirus disease 2019 (COVID-19) and dengue fever has been reported. Accurate and timely multiplex diagnosis is required to prevent future pandemics. Here, we developed an innovative microfluidic chip that enables a snapshot multiplex immunoassay for timely on-site response and offers unprecedented multiplexing capability with an operating procedure similar to that of lateral flow assays. An open microchannel assembly of individually engineered microbeads was developed to construct nine high-density test lines, which can be imaged in a 1 mm(2) field-of-view. Thus, simultaneous detection of multiple antibodies would be achievable in a single high-resolution snapshot. Next, we developed a novel pixel intensity-based imaging process to distinguish effective and non-specific fluorescence signals, thereby improving the reliability of this fluorescence-based immunoassay. Finally, the chip specifically identified and classified random combinations of arbovirus (Zika, dengue, and chikungunya viruses) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies within 30 min. Therefore, we believe that this snapshot multiplex immunoassay chip is a powerful diagnostic tool to control current and future pandemics. |*Biosensing Techniques[MESH] |*COVID-19[MESH] |*Zika Virus[MESH] |*Zika Virus Infection[MESH] |Humans[MESH] |Immunoassay[MESH] |Reproducibility of Results[MESH]A single snapshot multiplex immunoassay platform utilizing dense test (epmc).pdf DeepDyve Pubget Overpricing