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10.1016/j.xpro.2021.100617 http://scihub22266oqcxt.onion/10.1016/j.xpro.2021.100617 34095859!8164376!34095859     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 276.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       STAR+Protoc 2021 ; 2 (3): 100617 Nephropedia Template TP {{tp|p=34095859|t=2021. Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains |pdf=|usr=}}{{34095859}} gab.com Text Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains JO: STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34095859 #FOAvip This protocol is a comprehensive guide to phage display-based selection of virus neutralizing V(H) antibody domains. It details three optimized parts including (1) construction of a large-sized (theoretically > 10(11)) naive human antibody heavy chain domain library, (2) SARS-CoV-2 antigen expression and stable cell line construction, and (3) library panning for selection of SARS-CoV-2-specific antibody domains. Using this protocol, we identified a high-affinity neutralizing human V(H) antibody domain, V(H) ab8, which exhibits high prophylactic and therapeutic efficacy. For complete details on the use and execution of this protocol, please refer to Li et al. (2020). Twit Text FOAVip Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains ????STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34095859 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34095859 #FOAvip English Wikipedia <ref>{{Cite pmid|34095859}}</ref> Protocol for constructing large size human antibody heavy chain variable domain (V(H)) library and selection of SARS-CoV-2 neutralizing antibody domains #MMPMID34095859 Chen C; Sun Z; Liu X; Li W; Dimitrov DS STAR Protoc 2021[Sep]; 2 (3): 100617 PMID34095859show ga This protocol is a comprehensive guide to phage display-based selection of virus neutralizing V(H) antibody domains. It details three optimized parts including (1) construction of a large-sized (theoretically > 10(11)) naive human antibody heavy chain domain library, (2) SARS-CoV-2 antigen expression and stable cell line construction, and (3) library panning for selection of SARS-CoV-2-specific antibody domains. Using this protocol, we identified a high-affinity neutralizing human V(H) antibody domain, V(H) ab8, which exhibits high prophylactic and therapeutic efficacy. For complete details on the use and execution of this protocol, please refer to Li et al. (2020). |*Peptide Library[MESH] |Amino Acid Sequence[MESH] |Antibodies, Neutralizing/*immunology[MESH] |Antibodies, Viral/*immunology[MESH] |Base Sequence[MESH] |COVID-19/*immunology/virology[MESH] |Cell Surface Display Techniques/methods[MESH] |Humans[MESH] |Immunoglobulin Heavy Chains/*immunology[MESH] |Immunoglobulin Variable Region/*immunology[MESH] |SARS-CoV-2/*immunology/isolation & purification[MESH]Protocol for constructing large size human antibody heavy chain variab(epmc).pdf DeepDyve Pubget Overpricing