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10.1016/j.ab.2021.114267 http://scihub22266oqcxt.onion/10.1016/j.ab.2021.114267 34089699!ä!34089699 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Biochem 2021 ; 628 (ä): 114267 Nephropedia Template TP {{tp|p=34089699|t=2021. Inhibition of nonspecific polymerase activity using Poly(Aspartic) acid as a model anionic polyelectrolyte |pdf=|usr=}}{{34089699}} gab.com Text Inhibition of nonspecific polymerase activity using Poly(Aspartic) acid as a model anionic polyelectrolyte JO: Anal Biochem http://www.kidney.de/pget.php?&tw=1&pmid=34089699 #FOAvip DNA polymerases with strand-displacement activity allow to amplify nucleic acids under isothermal conditions but often lead to undesirable by-products. Here, we report the increase of specificity of isothermal amplification in the presence of poly (aspartic) acids (pAsp). We hypothesized that side reactions occur due to the binding of the phosphate backbone of synthesized DNA strands with surface amino groups of the polymerase, and weakly acidic polyelectrolytes could shield polymerase molecules from DNA and thereby inhibit nonspecific amplification. Suppression of nonspecific polymerase activity by pAsp was studied on multimerization as a model side reaction. It was found that a low concentration of pAsp (0.01%) provides successful amplification of specific DNA targets. The inhibitory effect of pAsp is due to its polymeric structure since aspartic acid did affect neither specific nor nonspecific amplification. Strongly acidic polyelectrolyte heparin does not possess the same selectivity since it suppresses any DNA synthesis. The applicability of pAsp to prevent nonspecific reactions and reliable detection of the specific target has been demonstrated on the genetic material of SARS-CoV-2 coronavirus using Loop-mediated isothermal amplification. Twit Text FOAVip Inhibition of nonspecific polymerase activity using Poly(Aspartic) acid as a model anionic polyelectrolyte ????Anal Biochem http://www.kidney.de/pget.php?&tw=1&pmid=34089699 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34089699 #FOAvip English Wikipedia <ref>{{Cite pmid|34089699}}</ref> Inhibition of nonspecific polymerase activity using Poly(Aspartic) acid as a model anionic polyelectrolyte #MMPMID34089699 Sakhabutdinova AR; Kamalov MI; Salakhieva DV; Mavzyutov AR; Garafutdinov RR Anal Biochem 2021[Sep]; 628 (ä): 114267 PMID34089699show ga DNA polymerases with strand-displacement activity allow to amplify nucleic acids under isothermal conditions but often lead to undesirable by-products. Here, we report the increase of specificity of isothermal amplification in the presence of poly (aspartic) acids (pAsp). We hypothesized that side reactions occur due to the binding of the phosphate backbone of synthesized DNA strands with surface amino groups of the polymerase, and weakly acidic polyelectrolytes could shield polymerase molecules from DNA and thereby inhibit nonspecific amplification. Suppression of nonspecific polymerase activity by pAsp was studied on multimerization as a model side reaction. It was found that a low concentration of pAsp (0.01%) provides successful amplification of specific DNA targets. The inhibitory effect of pAsp is due to its polymeric structure since aspartic acid did affect neither specific nor nonspecific amplification. Strongly acidic polyelectrolyte heparin does not possess the same selectivity since it suppresses any DNA synthesis. The applicability of pAsp to prevent nonspecific reactions and reliable detection of the specific target has been demonstrated on the genetic material of SARS-CoV-2 coronavirus using Loop-mediated isothermal amplification. |*COVID-19 Nucleic Acid Testing[MESH] |*Molecular Diagnostic Techniques[MESH] |*Nucleic Acid Amplification Techniques[MESH] |COVID-19/*genetics[MESH] |DNA-Directed DNA Polymerase/*chemistry[MESH] |Humans[MESH] |Peptides/*chemistry[MESH] |Polyelectrolytes/chemistry[MESH]Inhibition of nonspecific polymerase activity using Poly(Aspartic) aci(epmc).pdf DeepDyve Pubget Overpricing