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10.1038/s41598-021-91371-4 http://scihub22266oqcxt.onion/10.1038/s41598-021-91371-4 34088957!8178396!34088957     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 11885 Nephropedia Template TP {{tp|p=34088957|t=2021. Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions |pdf=|usr=}}{{34088957}} gab.com Text Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34088957 #FOAvip SARS-CoV-2 is an enveloped virus responsible for the Coronavirus Disease 2019 (COVID-19) pandemic. Here, single viruses were analyzed by atomic force microscopy (AFM) operating directly in a level 3 biosafety (BSL3) facility, which appeared as a fast and powerful method to assess at the nanoscale level and in 3D infectious virus morphology in its native conformation, or upon inactivation treatments. AFM imaging reveals structurally intact infectious and inactivated SARS-CoV-2 upon low concentration of formaldehyde treatment. This protocol combining AFM and plaque assays allows the preparation of intact inactivated SARS-CoV-2 particles for safe use of samples out of level 3 laboratory to accelerate researches against the COVID-19 pandemic. Overall, we illustrate how adapted BSL3-AFM is a remarkable toolbox for rapid and direct virus analysis based on nanoscale morphology. Twit Text FOAVip Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34088957 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34088957 #FOAvip English Wikipedia <ref>{{Cite pmid|34088957}}</ref> Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions #MMPMID34088957 Lyonnais S; Henaut M; Neyret A; Merida P; Cazevieille C; Gros N; Chable-Bessia C; Muriaux D Sci Rep 2021[Jun]; 11 (1): 11885 PMID34088957show ga SARS-CoV-2 is an enveloped virus responsible for the Coronavirus Disease 2019 (COVID-19) pandemic. Here, single viruses were analyzed by atomic force microscopy (AFM) operating directly in a level 3 biosafety (BSL3) facility, which appeared as a fast and powerful method to assess at the nanoscale level and in 3D infectious virus morphology in its native conformation, or upon inactivation treatments. AFM imaging reveals structurally intact infectious and inactivated SARS-CoV-2 upon low concentration of formaldehyde treatment. This protocol combining AFM and plaque assays allows the preparation of intact inactivated SARS-CoV-2 particles for safe use of samples out of level 3 laboratory to accelerate researches against the COVID-19 pandemic. Overall, we illustrate how adapted BSL3-AFM is a remarkable toolbox for rapid and direct virus analysis based on nanoscale morphology. |*Microscopy, Atomic Force[MESH] |Animals[MESH] |COVID-19/*virology[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH] |SARS-CoV-2/physiology/*ultrastructure[MESH] |Vero Cells[MESH] |Virion/physiology/*ultrastructure[MESH]Atomic force microscopy analysis of native infectious and inactivated (epmc).pdf DeepDyve Pubget Overpricing