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10.1016/j.jviromet.2021.114200 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114200 34087339!8166808!34087339     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 295 (ä): 114200 Nephropedia Template TP {{tp|p=34087339|t=2021. Comparison of eleven in vitro diagnostic assays for the detection of SARS-CoV-2 RNA |pdf=|usr=}}{{34087339}} gab.com Text Comparison of eleven in vitro diagnostic assays for the detection of SARS-CoV-2 RNA JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=34087339 #FOAvip Transmission mitigation of SARS-CoV-2 requires the availability of accurate and sensitive detection methods. There are several commercial ad hoc molecular diagnostic kits currently on the market, many of which have been evaluated by different groups. However, in low resource settings the availability and cost of these commercial kits can be a limiting factor for many diagnostic laboratories. In such cases alternatives need to be identified. With this in mind, eight commercial reverse transcription quantitative real-time PCR (RT-qPCR) master mixes from Applied Biosystems (Thermo Fisher Scientific), Bio-Rad, Biotech Rabbit, Promega, Qiagen, QuantaBio, Invitrogen (Thermo Fisher Scientific) and Takara using the same commercial primer and probe mix [LightMix(R) Modular SARS and Wuhan CoV E-gene mix (TIB MolBiol, Germany)] were evaluated. Three ad hoc molecular diagnostic kits [GeneFinder COVID-19 Plus RealAmp kit (Osang Healthcare); genesig(R) Real-Time PCR Coronavirus COVID-19 (Primerdesign); and ViroReal(R) Kit SARS-CoV-2 & SARS-CoV (Ingenetix)] were also included in the study. The limit of detection was calculated for each assay using serial dilutions of a defined clinical sample. The performances of the assays were compared using a panel of 178 clinical samples and their analytical specificity assessed against a panel of human betacoronaviruses. Inter assay agreement was assessed using statistical tests (Bland-Altman, Fleiss-Kappa and Cohen's Kappa) and was shown to be excellent to good in all cases. We conclude that all of the assays evaluated in this study can be used for the routine detection of SARS-CoV-2 and that the RT-qPCR master mixes are a valid alternative to ad hoc molecular diagnostic kits. Twit Text FOAVip Comparison of eleven in vitro diagnostic assays for the detection of SARS-CoV-2 RNA ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=34087339 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34087339 #FOAvip English Wikipedia <ref>{{Cite pmid|34087339}}</ref> Comparison of eleven in vitro diagnostic assays for the detection of SARS-CoV-2 RNA #MMPMID34087339 Dundon WG; Settypalli TBK; Spiegel K; Steinrigl A; Revilla-Fernandez S; Schmoll F; Naletoski I; Lamien CE; Cattoli G J Virol Methods 2021[Sep]; 295 (ä): 114200 PMID34087339show ga Transmission mitigation of SARS-CoV-2 requires the availability of accurate and sensitive detection methods. There are several commercial ad hoc molecular diagnostic kits currently on the market, many of which have been evaluated by different groups. However, in low resource settings the availability and cost of these commercial kits can be a limiting factor for many diagnostic laboratories. In such cases alternatives need to be identified. With this in mind, eight commercial reverse transcription quantitative real-time PCR (RT-qPCR) master mixes from Applied Biosystems (Thermo Fisher Scientific), Bio-Rad, Biotech Rabbit, Promega, Qiagen, QuantaBio, Invitrogen (Thermo Fisher Scientific) and Takara using the same commercial primer and probe mix [LightMix(R) Modular SARS and Wuhan CoV E-gene mix (TIB MolBiol, Germany)] were evaluated. Three ad hoc molecular diagnostic kits [GeneFinder COVID-19 Plus RealAmp kit (Osang Healthcare); genesig(R) Real-Time PCR Coronavirus COVID-19 (Primerdesign); and ViroReal(R) Kit SARS-CoV-2 & SARS-CoV (Ingenetix)] were also included in the study. The limit of detection was calculated for each assay using serial dilutions of a defined clinical sample. The performances of the assays were compared using a panel of 178 clinical samples and their analytical specificity assessed against a panel of human betacoronaviruses. Inter assay agreement was assessed using statistical tests (Bland-Altman, Fleiss-Kappa and Cohen's Kappa) and was shown to be excellent to good in all cases. We conclude that all of the assays evaluated in this study can be used for the routine detection of SARS-CoV-2 and that the RT-qPCR master mixes are a valid alternative to ad hoc molecular diagnostic kits. |*COVID-19 Nucleic Acid Testing[MESH] |*Reagent Kits, Diagnostic[MESH] |COVID-19/*diagnosis[MESH] |Diagnostic Tests, Routine[MESH] |Humans[MESH] |RNA, Viral/genetics[MESH] |Real-Time Polymerase Chain Reaction[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH]Comparison of eleven in vitro diagnostic assays for the detection of S(epmc).pdf DeepDyve Pubget Overpricing