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10.1111/imcb.12482 http://scihub22266oqcxt.onion/10.1111/imcb.12482 34086357!8242669!34086357     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Immunol+Cell+Biol 2021 ; 99 (9): 990-1000 Nephropedia Template TP {{tp|p=34086357|t=2021. SARS-CoV-2-specific CD8(+) T-cell responses and TCR signatures in the context of a prominent HLA-A*24:02 allomorph |pdf=|usr=}}{{34086357}} gab.com Text SARS-CoV-2-specific CD8(+) T-cell responses and TCR signatures in the context of a prominent HLA-A*24:02 allomorph JO: Immunol Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=34086357 #FOAvip In-depth understanding of human T-cell-mediated immunity in coronavirus disease 2019 (COVID-19) is needed if we are to optimize vaccine strategies and immunotherapies. Identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) T-cell epitopes and generation of peptide-human leukocyte antigen (peptide-HLA) tetramers facilitate direct ex vivo analyses of SARS-CoV-2-specific T cells and their T-cell receptor (TCR) repertoires. We utilized a combination of peptide prediction and in vitro peptide stimulation to validate novel SARS-CoV-2 epitopes restricted by HLA-A*24:02, one of the most prominent HLA class I alleles, especially in Indigenous and Asian populations. Of the peptides screened, three spike-derived peptides generated CD8(+) IFNgamma(+) responses above background, S(1208-1216) (QYIKWPWYI), S(448-456) (NYNYLYRLF) and S(193-201) (VFKNIDGYF), with S(1208) generating immunodominant CD8(+) IFNgamma(+) responses. Using peptide-HLA-I tetramers, we performed direct ex vivo tetramer enrichment for HLA-A*24:02-restricted CD8(+) T cells in COVID-19 patients and prepandemic controls. The precursor frequencies for HLA-A*24:02-restricted epitopes were within the range previously observed for other SARS-CoV-2 epitopes for both COVID-19 patients and prepandemic individuals. Naive A24/SARS-CoV-2-specific CD8(+) T cells increased nearly 7.5-fold above the average precursor frequency during COVID-19, gaining effector and memory phenotypes. Ex vivo single-cell analyses of TCRalphabeta repertoires found that the A24/S(448)(+) CD8(+) T-cell TCRalphabeta repertoire was driven by a common TCRbeta chain motif, whereas the A24/S(1208)(+) CD8(+) TCRalphabeta repertoire was diverse across COVID-19 patients. Our study provides an in depth characterization and important insights into SARS-CoV-2-specific CD8(+) T-cell responses associated with a prominent HLA-A*24:02 allomorph. This contributes to our knowledge on adaptive immune responses during primary COVID-19 and could be exploited in vaccine or immunotherapeutic approaches. Twit Text FOAVip SARS-CoV-2-specific CD8(+) T-cell responses and TCR signatures in the context of a prominent HLA-A*24:02 allomorph ????Immunol Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=34086357 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34086357 #FOAvip English Wikipedia <ref>{{Cite pmid|34086357}}</ref> SARS-CoV-2-specific CD8(+) T-cell responses and TCR signatures in the context of a prominent HLA-A*24:02 allomorph #MMPMID34086357 Rowntree LC; Petersen J; Juno JA; Chaurasia P; Wragg K; Koutsakos M; Hensen L; Wheatley AK; Kent SJ; Rossjohn J; Kedzierska K; Nguyen TH Immunol Cell Biol 2021[Oct]; 99 (9): 990-1000 PMID34086357show ga In-depth understanding of human T-cell-mediated immunity in coronavirus disease 2019 (COVID-19) is needed if we are to optimize vaccine strategies and immunotherapies. Identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) T-cell epitopes and generation of peptide-human leukocyte antigen (peptide-HLA) tetramers facilitate direct ex vivo analyses of SARS-CoV-2-specific T cells and their T-cell receptor (TCR) repertoires. We utilized a combination of peptide prediction and in vitro peptide stimulation to validate novel SARS-CoV-2 epitopes restricted by HLA-A*24:02, one of the most prominent HLA class I alleles, especially in Indigenous and Asian populations. Of the peptides screened, three spike-derived peptides generated CD8(+) IFNgamma(+) responses above background, S(1208-1216) (QYIKWPWYI), S(448-456) (NYNYLYRLF) and S(193-201) (VFKNIDGYF), with S(1208) generating immunodominant CD8(+) IFNgamma(+) responses. Using peptide-HLA-I tetramers, we performed direct ex vivo tetramer enrichment for HLA-A*24:02-restricted CD8(+) T cells in COVID-19 patients and prepandemic controls. The precursor frequencies for HLA-A*24:02-restricted epitopes were within the range previously observed for other SARS-CoV-2 epitopes for both COVID-19 patients and prepandemic individuals. Naive A24/SARS-CoV-2-specific CD8(+) T cells increased nearly 7.5-fold above the average precursor frequency during COVID-19, gaining effector and memory phenotypes. Ex vivo single-cell analyses of TCRalphabeta repertoires found that the A24/S(448)(+) CD8(+) T-cell TCRalphabeta repertoire was driven by a common TCRbeta chain motif, whereas the A24/S(1208)(+) CD8(+) TCRalphabeta repertoire was diverse across COVID-19 patients. Our study provides an in depth characterization and important insights into SARS-CoV-2-specific CD8(+) T-cell responses associated with a prominent HLA-A*24:02 allomorph. This contributes to our knowledge on adaptive immune responses during primary COVID-19 and could be exploited in vaccine or immunotherapeutic approaches. |*COVID-19/immunology[MESH] |*HLA-A24 Antigen[MESH] |CD8-Positive T-Lymphocytes/*immunology[MESH] |Humans[MESH] |Receptors, Antigen, T-Cell/*immunology[MESH]SARS-CoV-2-specific CD8(+) T-cell responses and TCR signatures in the (epmc).pdf DeepDyve Pubget Overpricing