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10.1038/s41598-021-91264-6

http://scihub22266oqcxt.onion/10.1038/s41598-021-91264-6
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34083682!8175367!34083682
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suck abstract from ncbi


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pmid34083682      Sci+Rep 2021 ; 11 (1): 11773
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  • Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution #MMPMID34083682
  • Colombo F; Corsiero E; Lewis MJ; Pitzalis C
  • Sci Rep 2021[Jun]; 11 (1): 11773 PMID34083682show ga
  • Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting classical restriction site (RS) sequence in human SARS-CoV-2 genomes and (2) comparing them with other recombinant SARS-CoV-like virus created for experimental purposes. Finally, we propose a novel approach consisting in the generation of a restriction endonucleases site map of SARS-CoV-2 and other related coronavirus genomes to be used as a fingerprint to trace the virus evolution.
  • |*Biological Evolution[MESH]
  • |Animals[MESH]
  • |Chiroptera/virology[MESH]
  • |DNA Barcoding, Taxonomic/*methods[MESH]
  • |DNA Restriction Enzymes/*genetics/metabolism[MESH]
  • |Genetic Markers[MESH]
  • |Genome, Viral[MESH]
  • |Humans[MESH]
  • |Restriction Mapping[MESH]
  • |SARS-CoV-2/*genetics/isolation & purification[MESH]


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