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10.1038/s41598-021-90766-7 http://scihub22266oqcxt.onion/10.1038/s41598-021-90766-7 34078924!8173023!34078924     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 11545 Nephropedia Template TP {{tp|p=34078924|t=2021. Covid-19 diagnosis by combining RT-PCR and pseudo-convolutional machines to characterize virus sequences |pdf=|usr=}}{{34078924}} gab.com Text Covid-19 diagnosis by combining RT-PCR and pseudo-convolutional machines to characterize virus sequences JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34078924 #FOAvip The Covid-19 pandemic, a disease transmitted by the SARS-CoV-2 virus, has already caused the infection of more than 120 million people, of which 70 million have been recovered, while 3 million people have died. The high speed of infection has led to the rapid depletion of public health resources in most countries. RT-PCR is Covid-19's reference diagnostic method. In this work we propose a new technique for representing DNA sequences: they are divided into smaller sequences with overlap in a pseudo-convolutional approach and represented by co-occurrence matrices. This technique eliminates multiple sequence alignment. Through the proposed method, it is possible to identify virus sequences from a large database: 347,363 virus DNA sequences from 24 virus families and SARS-CoV-2. When comparing SARS-CoV-2 with virus families with similar symptoms, we obtained [Formula: see text] for sensitivity and [Formula: see text] for specificity with MLP classifier and 30% overlap. When SARS-CoV-2 is compared to other coronaviruses and healthy human DNA sequences, we obtained [Formula: see text] for sensitivity and [Formula: see text] for specificity with MLP and 50% overlap. Therefore, the molecular diagnosis of Covid-19 can be optimized by combining RT-PCR and our pseudo-convolutional method to identify DNA sequences for SARS-CoV-2 with greater specificity and sensitivity. Twit Text FOAVip Covid-19 diagnosis by combining RT-PCR and pseudo-convolutional machines to characterize virus sequences ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34078924 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34078924 #FOAvip English Wikipedia <ref>{{Cite pmid|34078924}}</ref> Covid-19 diagnosis by combining RT-PCR and pseudo-convolutional machines to characterize virus sequences #MMPMID34078924 Gomes JC; Masood AI; Silva LHS; da Cruz Ferreira JRB; Freire Junior AA; Rocha ALDS; de Oliveira LCP; da Silva NRC; Fernandes BJT; Dos Santos WP Sci Rep 2021[Jun]; 11 (1): 11545 PMID34078924show ga The Covid-19 pandemic, a disease transmitted by the SARS-CoV-2 virus, has already caused the infection of more than 120 million people, of which 70 million have been recovered, while 3 million people have died. The high speed of infection has led to the rapid depletion of public health resources in most countries. RT-PCR is Covid-19's reference diagnostic method. In this work we propose a new technique for representing DNA sequences: they are divided into smaller sequences with overlap in a pseudo-convolutional approach and represented by co-occurrence matrices. This technique eliminates multiple sequence alignment. Through the proposed method, it is possible to identify virus sequences from a large database: 347,363 virus DNA sequences from 24 virus families and SARS-CoV-2. When comparing SARS-CoV-2 with virus families with similar symptoms, we obtained [Formula: see text] for sensitivity and [Formula: see text] for specificity with MLP classifier and 30% overlap. When SARS-CoV-2 is compared to other coronaviruses and healthy human DNA sequences, we obtained [Formula: see text] for sensitivity and [Formula: see text] for specificity with MLP and 50% overlap. Therefore, the molecular diagnosis of Covid-19 can be optimized by combining RT-PCR and our pseudo-convolutional method to identify DNA sequences for SARS-CoV-2 with greater specificity and sensitivity. |COVID-19 Nucleic Acid Testing/*methods[MESH] |Computational Biology/*methods[MESH] |DNA, Viral[MESH] |Humans[MESH] |Machine Learning[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2/*genetics[MESH] |Sensitivity and Specificity[MESH] |Support Vector Machine[MESH]Covid-19 diagnosis by combining RT-PCR and pseudo-convolutional machin(epmc).pdf DeepDyve Pubget Overpricing