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10.1186/s12985-021-01559-3 http://scihub22266oqcxt.onion/10.1186/s12985-021-01559-3 34078394!8170437!34078394     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virol+J 2021 ; 18 (1): 110 Nephropedia Template TP {{tp|p=34078394|t=2021. Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2 |pdf=|usr=}}{{34078394}} gab.com Text Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2 JO: Virol J http://www.kidney.de/pget.php?&tw=1&pmid=34078394 #FOAvip BACKGROUND: The reliable detection of SARS-CoV-2 has become one of the most important contributions to COVID-19 crisis management. With the publication of the first sequences of SARS-CoV-2, several diagnostic PCR assays have been developed and published. In addition to in-house assays the market was flooded with numerous commercially available ready-to-use PCR kits, with both approaches showing alarming shortages in reagent supply. AIM: Here we present a resource-efficient in-house protocol for the PCR detection of SARS-CoV-2 RNA in patient specimens (RKI/ZBS1 SARS-CoV-2 protocol). METHODS: Two duplex one-step real-time RT-PCR assays are run simultaneously and provide information on two different SARS-CoV-2 genomic regions. Each one is duplexed with a control that either indicates potential PCR inhibition or proves the successful extraction of nucleic acid from the clinical specimen. RESULTS: Limit of RNA detection for both SARS-CoV-2 assays is below 10 genomes per reaction. The protocol enables testing specimens in duplicate across the two different SARS-CoV-2 PCR assays, saving reagents by increasing testing capacity. The protocol can be run on various PCR cyclers with several PCR master mix kits. CONCLUSION: The presented RKI/ZBS1 SARS-CoV-2 protocol represents a cost-effective alternative in times of shortages when commercially available ready-to-use kits may not be available or affordable. Twit Text FOAVip Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2 ????Virol J http://www.kidney.de/pget.php?&tw=1&pmid=34078394 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34078394 #FOAvip English Wikipedia <ref>{{Cite pmid|34078394}}</ref> Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2 #MMPMID34078394 Michel J; Neumann M; Krause E; Rinner T; Muzeniek T; Grossegesse M; Hille G; Schwarz F; Puyskens A; Forster S; Biere B; Bourquain D; Domingo C; Brinkmann A; Schaade L; Schrick L; Nitsche A Virol J 2021[Jun]; 18 (1): 110 PMID34078394show ga BACKGROUND: The reliable detection of SARS-CoV-2 has become one of the most important contributions to COVID-19 crisis management. With the publication of the first sequences of SARS-CoV-2, several diagnostic PCR assays have been developed and published. In addition to in-house assays the market was flooded with numerous commercially available ready-to-use PCR kits, with both approaches showing alarming shortages in reagent supply. AIM: Here we present a resource-efficient in-house protocol for the PCR detection of SARS-CoV-2 RNA in patient specimens (RKI/ZBS1 SARS-CoV-2 protocol). METHODS: Two duplex one-step real-time RT-PCR assays are run simultaneously and provide information on two different SARS-CoV-2 genomic regions. Each one is duplexed with a control that either indicates potential PCR inhibition or proves the successful extraction of nucleic acid from the clinical specimen. RESULTS: Limit of RNA detection for both SARS-CoV-2 assays is below 10 genomes per reaction. The protocol enables testing specimens in duplicate across the two different SARS-CoV-2 PCR assays, saving reagents by increasing testing capacity. The protocol can be run on various PCR cyclers with several PCR master mix kits. CONCLUSION: The presented RKI/ZBS1 SARS-CoV-2 protocol represents a cost-effective alternative in times of shortages when commercially available ready-to-use kits may not be available or affordable. |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Coronavirus Envelope Proteins/genetics[MESH] |High-Throughput Nucleotide Sequencing/methods[MESH] |Humans[MESH] |Limit of Detection[MESH] |Polyproteins/genetics[MESH] |RNA, Viral/*analysis/genetics[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH] |Sensitivity and Specificity[MESH]Resource-efficient internally controlled in-house real-time PCR detect(epmc).pdf DeepDyve Pubget Overpricing