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10.1016/j.xcrm.2021.100319 http://scihub22266oqcxt.onion/10.1016/j.xcrm.2021.100319 34075364!8157118!34075364     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep+Med 2021 ; 2 (6): 100319 Nephropedia Template TP {{tp|p=34075364|t=2021. Intrinsic signal amplification by type III CRISPR-Cas systems provides a sequence-specific SARS-CoV-2 diagnostic |pdf=|usr=}}{{34075364}} gab.com Text Intrinsic signal amplification by type III CRISPR-Cas systems provides a sequence-specific SARS-CoV-2 diagnostic JO: Cell Rep Med http://www.kidney.de/pget.php?&tw=1&pmid=34075364 #FOAvip There is an urgent need for inexpensive new technologies that enable fast, reliable, and scalable detection of viruses. Here, we repurpose the type III CRISPR-Cas system for sensitive and sequence-specific detection of SARS-CoV-2. RNA recognition by the type III CRISPR complex triggers Cas10-mediated polymerase activity, which simultaneously generates pyrophosphates, protons, and cyclic oligonucleotides. We show that all three Cas10-polymerase products are detectable using colorimetric or fluorometric readouts. We design ten guide RNAs that target conserved regions of SARS-CoV-2 genomes. Multiplexing improves the sensitivity of amplification-free RNA detection from 10(7) copies/muL for a single guide RNA to 10(6) copies/muL for ten guides. To decrease the limit of detection to levels that are clinically relevant, we developed a two-pot reaction consisting of RT-LAMP followed by T7-transcription and type III CRISPR-based detection. The two-pot reaction has a sensitivity of 200 copies/muL and is completed using patient samples in less than 30 min. Twit Text FOAVip Intrinsic signal amplification by type III CRISPR-Cas systems provides a sequence-specific SARS-CoV-2 diagnostic ????Cell Rep Med http://www.kidney.de/pget.php?&tw=1&pmid=34075364 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34075364 #FOAvip English Wikipedia <ref>{{Cite pmid|34075364}}</ref> Intrinsic signal amplification by type III CRISPR-Cas systems provides a sequence-specific SARS-CoV-2 diagnostic #MMPMID34075364 Santiago-Frangos A; Hall LN; Nemudraia A; Nemudryi A; Krishna P; Wiegand T; Wilkinson RA; Snyder DT; Hedges JF; Cicha C; Lee HH; Graham A; Jutila MA; Taylor MP; Wiedenheft B Cell Rep Med 2021[Jun]; 2 (6): 100319 PMID34075364show ga There is an urgent need for inexpensive new technologies that enable fast, reliable, and scalable detection of viruses. Here, we repurpose the type III CRISPR-Cas system for sensitive and sequence-specific detection of SARS-CoV-2. RNA recognition by the type III CRISPR complex triggers Cas10-mediated polymerase activity, which simultaneously generates pyrophosphates, protons, and cyclic oligonucleotides. We show that all three Cas10-polymerase products are detectable using colorimetric or fluorometric readouts. We design ten guide RNAs that target conserved regions of SARS-CoV-2 genomes. Multiplexing improves the sensitivity of amplification-free RNA detection from 10(7) copies/muL for a single guide RNA to 10(6) copies/muL for ten guides. To decrease the limit of detection to levels that are clinically relevant, we developed a two-pot reaction consisting of RT-LAMP followed by T7-transcription and type III CRISPR-based detection. The two-pot reaction has a sensitivity of 200 copies/muL and is completed using patient samples in less than 30 min. |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |CRISPR-Cas Systems/*genetics[MESH] |Colorimetry[MESH] |Humans[MESH] |Molecular Diagnostic Techniques[MESH] |Nasopharynx/virology[MESH] |Nucleic Acid Amplification Techniques[MESH] |RNA, Guide, CRISPR-Cas Systems/metabolism[MESH] |RNA, Viral/chemistry/*metabolism[MESH]Intrinsic signal amplification by type III CRISPR-Cas systems provides(epmc).pdf DeepDyve Pubget Overpricing