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Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 NPJ+Sci+Food 2021 ; 5 (1): 12 Nephropedia Template TP
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Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine #MMPMID34075052
Zhang J; Fang X; Mao Y; Qi H; Wu J; Liu X; You F; Zhao W; Chen Y; Zheng L
NPJ Sci Food 2021[Jun]; 5 (1): 12 PMID34075052show ga
Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 x 10(-6) ng/mL in 20 s, with a wide linear range of 10(-5)-10(-1) ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.