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10.3390/ijms22094951 http://scihub22266oqcxt.onion/10.3390/ijms22094951 34066920!8125631!34066920     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Sci 2021 ; 22 (9): ä Nephropedia Template TP {{tp|p=34066920|t=2021. IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein |pdf=|usr=}}{{34066920}} gab.com Text IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=34066920 #FOAvip Diagnostic evaluation of specific antibodies against the SARS-CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein's immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101-222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provoke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design. Twit Text FOAVip IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=34066920 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34066920 #FOAvip English Wikipedia <ref>{{Cite pmid|34066920}}</ref> IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein #MMPMID34066920 Lopandic Z; Protic-Rosic I; Todorovic A; Glamoclija S; Gnjatovic M; Cujic D; Gavrovic-Jankulovic M Int J Mol Sci 2021[May]; 22 (9): ä PMID34066920show ga Diagnostic evaluation of specific antibodies against the SARS-CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein's immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101-222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provoke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design. |COVID-19/*blood/immunology[MESH] |Humans[MESH] |Immunoglobulin G/*immunology[MESH] |Immunoglobulin M/*immunology[MESH] |Recombinant Proteins/immunology[MESH] |SARS-CoV-2/*immunology[MESH] |Sensitivity and Specificity[MESH]IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein (epmc).pdf DeepDyve Pubget Overpricing