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10.1002/jmv.27113 http://scihub22266oqcxt.onion/10.1002/jmv.27113 34061367!8242665!34061367     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2021 ; 93 (10): 5816-5824 Nephropedia Template TP {{tp|p=34061367|t=2021. Longitudinal detection of SARS-CoV-2-specific antibody responses with different serological methods |pdf=|usr=}}{{34061367}} gab.com Text Longitudinal detection of SARS-CoV-2-specific antibody responses with different serological methods JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=34061367 #FOAvip Serological testing for anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies is used to detect ongoing or past SARS-CoV-2 infections. To study the kinetics of anti-SARS-CoV-2 antibodies and to assess the diagnostic performances of eight serological assays, we used 129 serum samples collected on known days post symptom onset (dpso) from 42 patients with polymerase chain reaction-confirmed coronavirus disease 2019 (COVID-19) and 54 serum samples from healthy blood donors, and children infected with seasonal coronaviruses. The sera were analyzed for the presence of immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA) antibodies using indirect immunofluorescence testing (IIFT) based on SARS-CoV-2-infected cells. They were further tested for antibodies against the S1 domain of the SARS-CoV-2 spike protein (IgG, IgA) and against the viral nucleocapsid protein (IgG, IgM) using enzyme-linked immunosorbent assays. The assay specificities were 94.4%-100%. The sensitivities varied largely between assays, reflecting their respective purposes. The sensitivities of IgA and IgM assays were the highest between 11 and 20 dpso, whereas the sensitivities of IgG assays peaked between 20 and 60 dpso. IIFT showed the highest sensitivities due to the use of the whole SARS-CoV-2 as substrate and provided information on whether or not the individual has been infected with SARS-CoV-2. Enzyme-linked immunosorbent assays provided further information about both the prevalence and concentration of specific antibodies against selected antigens of SARS-CoV-2. Twit Text FOAVip Longitudinal detection of SARS-CoV-2-specific antibody responses with different serological methods ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=34061367 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34061367 #FOAvip English Wikipedia <ref>{{Cite pmid|34061367}}</ref> Longitudinal detection of SARS-CoV-2-specific antibody responses with different serological methods #MMPMID34061367 Emmerich P; von Possel R; Hemmer CJ; Fritzsche C; Geerdes-Fenge H; Menge B; Messing C; Borchardt-Loholter V; Deschermeier C; Steinhagen K J Med Virol 2021[Oct]; 93 (10): 5816-5824 PMID34061367show ga Serological testing for anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies is used to detect ongoing or past SARS-CoV-2 infections. To study the kinetics of anti-SARS-CoV-2 antibodies and to assess the diagnostic performances of eight serological assays, we used 129 serum samples collected on known days post symptom onset (dpso) from 42 patients with polymerase chain reaction-confirmed coronavirus disease 2019 (COVID-19) and 54 serum samples from healthy blood donors, and children infected with seasonal coronaviruses. The sera were analyzed for the presence of immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA) antibodies using indirect immunofluorescence testing (IIFT) based on SARS-CoV-2-infected cells. They were further tested for antibodies against the S1 domain of the SARS-CoV-2 spike protein (IgG, IgA) and against the viral nucleocapsid protein (IgG, IgM) using enzyme-linked immunosorbent assays. The assay specificities were 94.4%-100%. The sensitivities varied largely between assays, reflecting their respective purposes. The sensitivities of IgA and IgM assays were the highest between 11 and 20 dpso, whereas the sensitivities of IgG assays peaked between 20 and 60 dpso. IIFT showed the highest sensitivities due to the use of the whole SARS-CoV-2 as substrate and provided information on whether or not the individual has been infected with SARS-CoV-2. Enzyme-linked immunosorbent assays provided further information about both the prevalence and concentration of specific antibodies against selected antigens of SARS-CoV-2. |*COVID-19 Serological Testing[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Antibodies, Viral/*blood/immunology[MESH] |COVID-19/*blood/diagnosis[MESH] |Coronavirus Nucleocapsid Proteins/immunology[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Female[MESH] |Fluorescent Antibody Technique, Indirect[MESH] |Humans[MESH] |Immunoglobulin Isotypes/blood[MESH] |Kinetics[MESH] |Male[MESH] |Middle Aged[MESH] |Phosphoproteins/immunology[MESH] |SARS-CoV-2/*immunology/isolation & purification[MESH] |Sensitivity and Specificity[MESH]Longitudinal detection of SARS-CoV-2-specific antibody responses with (epmc).pdf DeepDyve Pubget Overpricing