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10.1016/j.virol.2021.05.009 http://scihub22266oqcxt.onion/10.1016/j.virol.2021.05.009 34052578!8144927!34052578     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virology 2021 ; 560 (ä): 110-115 Nephropedia Template TP {{tp|p=34052578|t=2021. Azelastine inhibits viropexis of SARS-CoV-2 spike pseudovirus by binding to SARS-CoV-2 entry receptor ACE2 |pdf=|usr=}}{{34052578}} gab.com Text Azelastine inhibits viropexis of SARS-CoV-2 spike pseudovirus by binding to SARS-CoV-2 entry receptor ACE2 JO: Virology http://www.kidney.de/pget.php?&tw=1&pmid=34052578 #FOAvip A recent study have reported that pre-use of azelastine is associated with a decrease in COVID-19 positive test results among susceptible elderly people. Besides, it has been reported that antihistamine drugs could prevent viruses from entering cells. The purpose of this study is to investigate whether azelastine have antiviral activity against SARS-CoV-2 in vitro and the possible mechanism. Here, we discovered antihistamine azelastine has an affinity to ACE2 by cell membrane chromatography (CMC); Then we determined the equilibrium dissociation constant (K(D)) of azelastine-ACE2 as (2.58 +/- 0.48) x 10(-7) M by surface plasmon resonance (SPR). The results of molecular docking showed that azelastine could form an obvious hydrogen bond with Lys353. The pseudovirus infection experiments showed that azelastine effectively inhibited viral entry (EC(50) = 3.834 muM). Our work provides a new perspective for the screening method of drug repositioning for COVID-19, and an attractive and promising drug candidate for anti-SARS-CoV-2. Twit Text FOAVip Azelastine inhibits viropexis of SARS-CoV-2 spike pseudovirus by binding to SARS-CoV-2 entry receptor ACE2 ????Virology http://www.kidney.de/pget.php?&tw=1&pmid=34052578 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34052578 #FOAvip English Wikipedia <ref>{{Cite pmid|34052578}}</ref> Azelastine inhibits viropexis of SARS-CoV-2 spike pseudovirus by binding to SARS-CoV-2 entry receptor ACE2 #MMPMID34052578 Ge S; Lu J; Hou Y; Lv Y; Wang C; He H Virology 2021[Aug]; 560 (ä): 110-115 PMID34052578show ga A recent study have reported that pre-use of azelastine is associated with a decrease in COVID-19 positive test results among susceptible elderly people. Besides, it has been reported that antihistamine drugs could prevent viruses from entering cells. The purpose of this study is to investigate whether azelastine have antiviral activity against SARS-CoV-2 in vitro and the possible mechanism. Here, we discovered antihistamine azelastine has an affinity to ACE2 by cell membrane chromatography (CMC); Then we determined the equilibrium dissociation constant (K(D)) of azelastine-ACE2 as (2.58 +/- 0.48) x 10(-7) M by surface plasmon resonance (SPR). The results of molecular docking showed that azelastine could form an obvious hydrogen bond with Lys353. The pseudovirus infection experiments showed that azelastine effectively inhibited viral entry (EC(50) = 3.834 muM). Our work provides a new perspective for the screening method of drug repositioning for COVID-19, and an attractive and promising drug candidate for anti-SARS-CoV-2. |Angiotensin-Converting Enzyme 2/*metabolism[MESH] |Antiviral Agents/metabolism/*pharmacology[MESH] |Cell Membrane/metabolism[MESH] |Chromatography, Affinity[MESH] |Drug Repositioning[MESH] |HEK293 Cells[MESH] |Histamine H1 Antagonists, Non-Sedating/metabolism/pharmacology[MESH] |Humans[MESH] |Molecular Docking Simulation[MESH] |Phthalazines/metabolism/*pharmacology[MESH] |Protein Binding[MESH] |SARS-CoV-2[MESH] |Spike Glycoprotein, Coronavirus/*antagonists & inhibitors/metabolism[MESH]Azelastine inhibits viropexis of SARS-CoV-2 spike pseudovirus by bindi(epmc).pdf DeepDyve Pubget Overpricing