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10.1016/j.jchromb.2021.122768 http://scihub22266oqcxt.onion/10.1016/j.jchromb.2021.122768 34052564!8133798!34052564     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Chromatogr+B+Analyt+Technol+Biomed+Life+Sci 2021 ; 1176 (ä): 122768 Nephropedia Template TP {{tp|p=34052564|t=2021. Development and validation of a sensitive, fast and simple LC-MS / MS method for the quantitation of favipiravir in human serum |pdf=|usr=}}{{34052564}} gab.com Text Development and validation of a sensitive, fast and simple LC-MS / MS method for the quantitation of favipiravir in human serum JO: J Chromatogr B Analyt Technol Biomed Life Sci http://www.kidney.de/pget.php?&tw=1&pmid=34052564 #FOAvip Favipiravir is a broad-spectrum inhibitor of viral RNA polymerase. It is currently used as a possible treatment for coronavirus disease 2019 (COVID-19). Pre-clinical or clinical trials of favipiravir require robust, sensitive, and accurate bioanalytical methods for quantitation of favipiravir levels. Recently, several studies have been reported about developing a validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for measuring favipiravir levels. However, these methods were validated predominantly for plasma samples, electrospray ionization was operated only in negative or positive mode, and clinical application of these methods has not been applied for patients with COVID-19. This study aimed was to develop a validated LC-MS/MS method for the measurement of favipiravir levels in positive and negative electrospray ionization mode and to perform a pilot study in patients with COVID-19 receiving favipiravir to demonstrate the applicability of this method in biological samples. Simple protein precipitation was used for the extraction of favipiravir from the desired matrix. Favipiravir levels were quantitated using MS / MS with an electrospray ionization source in positive and negative multiple reaction monitoring (MRM) mode. The chromatographic detection was performed on a reverse-phase Phenomenex C18 column (50 mm x 4.6 mm, 5 microm, 100 A) with gradient elution using 0.1% formic acid in water and 0.1% formic acid in methanol as mobile phase. The method was linear over the concentration ranges of 0.048-50 microg/mL (in negative ionization mode) and 0.062-50 microg/mL (in positive ionization mode) with a correlation coefficient (r(2)) better than 0.998. The total run time was 3.5 min. The intra-assay and inter-assay %CV values were less than 7.2% and 8.0%, respectively. A simple, rapid and robust LC-MS / MS method was developed for the measurement of favipiravir and validation studies were performed. The validated method was successfully applied for drug level measurement in COVID-19 patients receiving favipiravir. Twit Text FOAVip Development and validation of a sensitive, fast and simple LC-MS / MS method for the quantitation of favipiravir in human serum ????J Chromatogr B Analyt Technol Biomed Life Sci http://www.kidney.de/pget.php?&tw=1&pmid=34052564 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34052564 #FOAvip English Wikipedia <ref>{{Cite pmid|34052564}}</ref> Development and validation of a sensitive, fast and simple LC-MS / MS method for the quantitation of favipiravir in human serum #MMPMID34052564 Eryavuz Onmaz D; Abusoglu S; Onmaz M; Yerlikaya FH; Unlu A J Chromatogr B Analyt Technol Biomed Life Sci 2021[Jun]; 1176 (ä): 122768 PMID34052564show ga Favipiravir is a broad-spectrum inhibitor of viral RNA polymerase. It is currently used as a possible treatment for coronavirus disease 2019 (COVID-19). Pre-clinical or clinical trials of favipiravir require robust, sensitive, and accurate bioanalytical methods for quantitation of favipiravir levels. Recently, several studies have been reported about developing a validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for measuring favipiravir levels. However, these methods were validated predominantly for plasma samples, electrospray ionization was operated only in negative or positive mode, and clinical application of these methods has not been applied for patients with COVID-19. This study aimed was to develop a validated LC-MS/MS method for the measurement of favipiravir levels in positive and negative electrospray ionization mode and to perform a pilot study in patients with COVID-19 receiving favipiravir to demonstrate the applicability of this method in biological samples. Simple protein precipitation was used for the extraction of favipiravir from the desired matrix. Favipiravir levels were quantitated using MS / MS with an electrospray ionization source in positive and negative multiple reaction monitoring (MRM) mode. The chromatographic detection was performed on a reverse-phase Phenomenex C18 column (50 mm x 4.6 mm, 5 microm, 100 A) with gradient elution using 0.1% formic acid in water and 0.1% formic acid in methanol as mobile phase. The method was linear over the concentration ranges of 0.048-50 microg/mL (in negative ionization mode) and 0.062-50 microg/mL (in positive ionization mode) with a correlation coefficient (r(2)) better than 0.998. The total run time was 3.5 min. The intra-assay and inter-assay %CV values were less than 7.2% and 8.0%, respectively. A simple, rapid and robust LC-MS / MS method was developed for the measurement of favipiravir and validation studies were performed. The validated method was successfully applied for drug level measurement in COVID-19 patients receiving favipiravir. |*COVID-19 Drug Treatment[MESH] |Amides/administration & dosage/*blood/therapeutic use[MESH] |Antiviral Agents/administration & dosage/blood/therapeutic use[MESH] |COVID-19/blood[MESH] |Chromatography, Liquid/*methods[MESH] |Drug Stability[MESH] |Humans[MESH] |Limit of Detection[MESH] |Pyrazines/administration & dosage/*blood/therapeutic use[MESH] |Sensitivity and Specificity[MESH] |Spectrometry, Mass, Electrospray Ionization/methods[MESH]Development and validation of a sensitive, fast and simple LC-MS / MS (epmc).pdf DeepDyve Pubget Overpricing