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10.1016/j.jviromet.2021.114185 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114185 34051244!8149472!34051244     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 295 (ä): 114185 Nephropedia Template TP {{tp|p=34051244|t=2021. Digital PCR assay for the effective detection of COVID-19 patients with SARS-CoV-2 low viral load |pdf=|usr=}}{{34051244}} gab.com Text Digital PCR assay for the effective detection of COVID-19 patients with SARS-CoV-2 low viral load JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=34051244 #FOAvip OBJECTIVE: Viral nucleic acid detection by real-time reverse transcription polymerase chain reaction (qPCR) is the current standard method for diagnosis of SARS-CoV-2 infection. However, due to low viral load in some COVID-19 patients, false negative results from this method have been repeatedly reported. METHOD: In this study, we compared the sensitivity and specificity of digital PCR (dPCR) in simulated samples and clinical samples with qPCR assay through a series of vigorous tests. RESULTS: The results showed that dPCR was more sensitive than qPCR especially for samples with low viral load (</=3 copies). In addition, dPCR had similar specificity as qPCR and could effectively distinguish other human coronaviruses and influenza virus from SARS-CoV-2. More importantly, dPCR was more sensitive than qPCR in detecting the virus in the "negative" samples from recurrent COVID-19 patients. CONCLUSIONS: In summary, dPCR could serve as a powerful complement to the current qPCR method for SARS-CoV-2 detection, especially for the samples with extremely low viral load, such as recurrent COVID-19 patients. Twit Text FOAVip Digital PCR assay for the effective detection of COVID-19 patients with SARS-CoV-2 low viral load ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=34051244 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34051244 #FOAvip English Wikipedia <ref>{{Cite pmid|34051244}}</ref> Digital PCR assay for the effective detection of COVID-19 patients with SARS-CoV-2 low viral load #MMPMID34051244 Sun Y; Ding C; Chen Q; Xie J; Yu J; Shi Y; Jiang C; Zhang Z; He H; Ge Y; Li W; He J; Gao Y J Virol Methods 2021[Sep]; 295 (ä): 114185 PMID34051244show ga OBJECTIVE: Viral nucleic acid detection by real-time reverse transcription polymerase chain reaction (qPCR) is the current standard method for diagnosis of SARS-CoV-2 infection. However, due to low viral load in some COVID-19 patients, false negative results from this method have been repeatedly reported. METHOD: In this study, we compared the sensitivity and specificity of digital PCR (dPCR) in simulated samples and clinical samples with qPCR assay through a series of vigorous tests. RESULTS: The results showed that dPCR was more sensitive than qPCR especially for samples with low viral load ( |*Viral Load[MESH] |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |Humans[MESH] |RNA, Viral/genetics[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |Recurrence[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH]Digital PCR assay for the effective detection of COVID-19 patients wit(epmc).pdf DeepDyve Pubget Overpricing