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10.1016/j.jim.2021.113082 http://scihub22266oqcxt.onion/10.1016/j.jim.2021.113082 34051226!8152238!34051226     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Immunol+Methods 2021 ; 495 (ä): 113082 Nephropedia Template TP {{tp|p=34051226|t=2021. Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies |pdf=|usr=}}{{34051226}} gab.com Text Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies JO: J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=34051226 #FOAvip The development of new diagnostic assays become a priority for managing COVID-19. To this aim, we presented here an in-house ELISA based on the production of two major recombinant and high-quality antigens from SARS-CoV-2. Full-length N and S-RBD fragment proteins fused to mouse IgG2a-Fc were produced in the CHO cell line. Secreted recombinant proteins were easily purified with standard Protein A chromatography and were used in an in-house ELISA to detect anti-N and anti-RBD IgGs in the plasma of COVID-19 RTPCR-positive patients. High reactivity against recombinant antigens was readily detected in all positive plasma samples, whereas no recognition was observed with control healthy subject's plasmas. Remarkably, unpurified recombinant N protein obtained from cell culture supernatant was also suitable for the monitoring by ELISA of IgG levels in positive patients. This work provides an early prospection for low price but high-quality serological kit development. Twit Text FOAVip Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies ????J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=34051226 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34051226 #FOAvip English Wikipedia <ref>{{Cite pmid|34051226}}</ref> Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies #MMPMID34051226 Frumence E; Lebeau G; Viranaicken W; Dobi A; Vagner D; Lalarizo Rakoto M; Sandenon Seteyen AL; Giry C; Septembre-Malaterre A; Raffray L; Gasque P J Immunol Methods 2021[Aug]; 495 (ä): 113082 PMID34051226show ga The development of new diagnostic assays become a priority for managing COVID-19. To this aim, we presented here an in-house ELISA based on the production of two major recombinant and high-quality antigens from SARS-CoV-2. Full-length N and S-RBD fragment proteins fused to mouse IgG2a-Fc were produced in the CHO cell line. Secreted recombinant proteins were easily purified with standard Protein A chromatography and were used in an in-house ELISA to detect anti-N and anti-RBD IgGs in the plasma of COVID-19 RTPCR-positive patients. High reactivity against recombinant antigens was readily detected in all positive plasma samples, whereas no recognition was observed with control healthy subject's plasmas. Remarkably, unpurified recombinant N protein obtained from cell culture supernatant was also suitable for the monitoring by ELISA of IgG levels in positive patients. This work provides an early prospection for low price but high-quality serological kit development. |Animals[MESH] |Antibodies, Viral/blood[MESH] |CHO Cells[MESH] |COVID-19 Serological Testing/economics/*methods[MESH] |COVID-19/*immunology[MESH] |Costs and Cost Analysis[MESH] |Cricetulus[MESH] |Enzyme-Linked Immunosorbent Assay/*methods[MESH] |Humans[MESH] |Immunoglobulin Fc Fragments/genetics/*metabolism[MESH] |Immunoglobulin G/genetics/*metabolism[MESH] |Recombinant Proteins/genetics/*metabolism[MESH] |SARS-CoV-2/*physiology[MESH] |Sensitivity and Specificity[MESH]Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins (epmc).pdf DeepDyve Pubget Overpricing