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10.1016/j.cmi.2021.05.025 http://scihub22266oqcxt.onion/10.1016/j.cmi.2021.05.025 34044153!8142743!34044153     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Microbiol+Infect 2021 ; 27 (9): 1353.e1-1353.e5 Nephropedia Template TP {{tp|p=34044153|t=2021. SARS-CoV-2 N gene dropout and N gene Ct value shift as indicator for the presence of B 1 1 7 lineage in a commercial multiplex PCR assay |pdf=|usr=}}{{34044153}} gab.com Text SARS-CoV-2 N gene dropout and N gene Ct value shift as indicator for the presence of B 1 1 7 lineage in a commercial multiplex PCR assay JO: Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=34044153 #FOAvip OBJECTIVES: Detection and surveillance of SARS-CoV-2 is of eminent importance, particularly due to the rapid emergence of variants of concern (VOCs). In this study we evaluated if a commercially available quantitative real-time PCR (qRT-PCR) assay can identify SARS-CoV-2 B.1.1.7 lineage samples by a specific N gene dropout or Ct value shift compared with the S or RdRp gene. METHODS: VOC B.1.1.7 and non-B.1.1.7 SARS-CoV-2-positive patient samples were identified via whole-genome sequencing and variant-specific PCR. Confirmed B.1.1.7 (n = 48) and non-B.1.1.7 samples (n = 58) were analysed using the Allplex SARS-CoV-2/FluA/FluB/RSV PCR assay for presence of SARS-CoV-2 S, RdRp and N genes. The N gene coding sequence of SARS-CoV-2 with and without the D3L mutation (specific for B.1.1.7) was cloned into pCRII-TOPO vectors to validate polymorphism-dependent N gene dropout with the Allplex SARS-CoV-2/FluA/FluB/RSV PCR assay. RESULTS: All studied B.1.1.7-positive patient samples showed significantly higher Ct values in qRT-PCR (Delta6-10, N gene dropout on Ct values > 29) of N gene than the corresponding values of S (p </= 0.0001) and RdRp (p </= 0.0001) genes. The assay reliably discriminated B.1.1.7 and non-B.1.1.7 positive samples (area under the curve = 1) in a receiver operating characteristic curve analysis. Identical Ct value shifts (Delta7-10) were detected in reverse genetic experiments, using isolated plasmids containing N gene coding sequences corresponding to D3 or 3L variants. DISCUSSION: An N gene dropout or Ct value shift is shown for B.1.1.7-positive samples in the Allplex SARS-CoV-2/FluA/FluB/RSV PCR assay. This approach can be used as a rapid tool for B.1.1.7 detection in single assay high throughput diagnostics. Twit Text FOAVip SARS-CoV-2 N gene dropout and N gene Ct value shift as indicator for the presence of B 1 1 7 lineage in a commercial multiplex PCR assay ????Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=34044153 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34044153 #FOAvip English Wikipedia <ref>{{Cite pmid|34044153}}</ref> SARS-CoV-2 N gene dropout and N gene Ct value shift as indicator for the presence of B 1 1 7 lineage in a commercial multiplex PCR assay #MMPMID34044153 Wollschlager P; Todt D; Gerlitz N; Pfaender S; Bollinger T; Sing A; Dangel A; Ackermann N; Korn K; Ensser A; Steinmann E; Buhl M; Steinmann J Clin Microbiol Infect 2021[Sep]; 27 (9): 1353.e1-1353.e5 PMID34044153show ga OBJECTIVES: Detection and surveillance of SARS-CoV-2 is of eminent importance, particularly due to the rapid emergence of variants of concern (VOCs). In this study we evaluated if a commercially available quantitative real-time PCR (qRT-PCR) assay can identify SARS-CoV-2 B.1.1.7 lineage samples by a specific N gene dropout or Ct value shift compared with the S or RdRp gene. METHODS: VOC B.1.1.7 and non-B.1.1.7 SARS-CoV-2-positive patient samples were identified via whole-genome sequencing and variant-specific PCR. Confirmed B.1.1.7 (n = 48) and non-B.1.1.7 samples (n = 58) were analysed using the Allplex SARS-CoV-2/FluA/FluB/RSV PCR assay for presence of SARS-CoV-2 S, RdRp and N genes. The N gene coding sequence of SARS-CoV-2 with and without the D3L mutation (specific for B.1.1.7) was cloned into pCRII-TOPO vectors to validate polymorphism-dependent N gene dropout with the Allplex SARS-CoV-2/FluA/FluB/RSV PCR assay. RESULTS: All studied B.1.1.7-positive patient samples showed significantly higher Ct values in qRT-PCR (Delta6-10, N gene dropout on Ct values > 29) of N gene than the corresponding values of S (p |COVID-19 Nucleic Acid Testing[MESH] |COVID-19/*diagnosis[MESH] |Coronavirus Nucleocapsid Proteins/*genetics[MESH] |Genome, Viral[MESH] |High-Throughput Nucleotide Sequencing[MESH] |Humans[MESH] |Multiplex Polymerase Chain Reaction[MESH] |Mutation[MESH] |ROC Curve[MESH] |SARS-CoV-2/*classification/genetics[MESH] |Sensitivity and Specificity[MESH]SARS-CoV-2 N gene dropout and N gene Ct value shift as indicator for t(epmc).pdf DeepDyve Pubget Overpricing