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10.1038/s41467-021-22664-5 http://scihub22266oqcxt.onion/10.1038/s41467-021-22664-5 34035246!8149640!34035246     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 3132 Nephropedia Template TP {{tp|p=34035246|t=2021. Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq |pdf=|usr=}}{{34035246}} gab.com Text Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34035246 #FOAvip The COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective tests monitoring viral spread. Here we present SARSeq, saliva analysis by RNA sequencing, a method to detect SARS-CoV-2 and other respiratory viruses on tens of thousands of samples in parallel. SARSeq relies on next generation sequencing of multiple amplicons generated in a multiplexed RT-PCR reaction. Two-dimensional, unique dual indexing, using four indices per sample, enables unambiguous and scalable assignment of reads to individual samples. We calibrate SARSeq on SARS-CoV-2 synthetic RNA, virions, and hundreds of human samples of various types. Robustness and sensitivity were virtually identical to quantitative RT-PCR. Double-blinded benchmarking to gold standard quantitative-RT-PCR performed by human diagnostics laboratories confirms this high sensitivity. SARSeq can be used to detect Influenza A and B viruses and human rhinovirus in parallel, and can be expanded for detection of other pathogens. Thus, SARSeq is ideally suited for differential diagnostic of infections during a pandemic. Twit Text FOAVip Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34035246 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34035246 #FOAvip English Wikipedia <ref>{{Cite pmid|34035246}}</ref> Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq #MMPMID34035246 Yelagandula R; Bykov A; Vogt A; Heinen R; Ozkan E; Strobl MM; Baar JC; Uzunova K; Hajdusits B; Kordic D; Suljic E; Kurtovic-Kozaric A; Izetbegovic S; Schaeffer J; Hufnagl P; Zoufaly A; Seitz T; Fodinger M; Allerberger F; Stark A; Cochella L; Elling U Nat Commun 2021[May]; 12 (1): 3132 PMID34035246show ga The COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective tests monitoring viral spread. Here we present SARSeq, saliva analysis by RNA sequencing, a method to detect SARS-CoV-2 and other respiratory viruses on tens of thousands of samples in parallel. SARSeq relies on next generation sequencing of multiple amplicons generated in a multiplexed RT-PCR reaction. Two-dimensional, unique dual indexing, using four indices per sample, enables unambiguous and scalable assignment of reads to individual samples. We calibrate SARSeq on SARS-CoV-2 synthetic RNA, virions, and hundreds of human samples of various types. Robustness and sensitivity were virtually identical to quantitative RT-PCR. Double-blinded benchmarking to gold standard quantitative-RT-PCR performed by human diagnostics laboratories confirms this high sensitivity. SARSeq can be used to detect Influenza A and B viruses and human rhinovirus in parallel, and can be expanded for detection of other pathogens. Thus, SARSeq is ideally suited for differential diagnostic of infections during a pandemic. |*Clinical Laboratory Techniques[MESH] |*High-Throughput Screening Assays[MESH] |COVID-19/diagnosis[MESH] |Diagnosis, Differential[MESH] |High-Throughput Nucleotide Sequencing[MESH] |Humans[MESH] |Polymerase Chain Reaction[MESH] |RNA, Viral/genetics[MESH] |Respiratory Tract Infections/*diagnosis/virology[MESH] |SARS-CoV-2/genetics/isolation & purification[MESH] |Saliva/virology[MESH] |Sensitivity and Specificity[MESH] |Viral Proteins/genetics[MESH]Multiplexed detection of SARS-CoV-2 and other respiratory infections i(epmc).pdf DeepDyve Pubget Overpricing