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10.1016/j.immuni.2021.05.001 http://scihub22266oqcxt.onion/10.1016/j.immuni.2021.05.001 34022127!8101792!34022127     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Immunity 2021 ; 54 (6): 1290-1303.e7 Nephropedia Template TP {{tp|p=34022127|t=2021. Profiling B cell immunodominance after SARS-CoV-2 infection reveals antibody evolution to non-neutralizing viral targets |pdf=|usr=}}{{34022127}} gab.com Text Profiling B cell immunodominance after SARS-CoV-2 infection reveals antibody evolution to non-neutralizing viral targets JO: Immunity http://www.kidney.de/pget.php?&tw=1&pmid=34022127 #FOAvip Dissecting the evolution of memory B cells (MBCs) against SARS-CoV-2 is critical for understanding antibody recall upon secondary exposure. Here, we used single-cell sequencing to profile SARS-CoV-2-reactive B cells in 38 COVID-19 patients. Using oligo-tagged antigen baits, we isolated B cells specific to the SARS-CoV-2 spike, nucleoprotein (NP), open reading frame 8 (ORF8), and endemic human coronavirus (HCoV) spike proteins. SARS-CoV-2 spike-specific cells were enriched in the memory compartment of acutely infected and convalescent patients several months post symptom onset. With severe acute infection, substantial populations of endemic HCoV-reactive antibody-secreting cells were identified and possessed highly mutated variable genes, signifying preexisting immunity. Finally, MBCs exhibited pronounced maturation to NP and ORF8 over time, especially in older patients. Monoclonal antibodies against these targets were non-neutralizing and non-protective in vivo. These findings reveal antibody adaptation to non-neutralizing intracellular antigens during infection, emphasizing the importance of vaccination for inducing neutralizing spike-specific MBCs. Twit Text FOAVip Profiling B cell immunodominance after SARS-CoV-2 infection reveals antibody evolution to non-neutralizing viral targets ????Immunity http://www.kidney.de/pget.php?&tw=1&pmid=34022127 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34022127 #FOAvip English Wikipedia <ref>{{Cite pmid|34022127}}</ref> Profiling B cell immunodominance after SARS-CoV-2 infection reveals antibody evolution to non-neutralizing viral targets #MMPMID34022127 Dugan HL; Stamper CT; Li L; Changrob S; Asby NW; Halfmann PJ; Zheng NY; Huang M; Shaw DG; Cobb MS; Erickson SA; Guthmiller JJ; Stovicek O; Wang J; Winkler ES; Madariaga ML; Shanmugarajah K; Jansen MO; Amanat F; Stewart I; Utset HA; Huang J; Nelson CA; Dai YN; Hall PD; Jedrzejczak RP; Joachimiak A; Krammer F; Diamond MS; Fremont DH; Kawaoka Y; Wilson PC Immunity 2021[Jun]; 54 (6): 1290-1303.e7 PMID34022127show ga Dissecting the evolution of memory B cells (MBCs) against SARS-CoV-2 is critical for understanding antibody recall upon secondary exposure. Here, we used single-cell sequencing to profile SARS-CoV-2-reactive B cells in 38 COVID-19 patients. Using oligo-tagged antigen baits, we isolated B cells specific to the SARS-CoV-2 spike, nucleoprotein (NP), open reading frame 8 (ORF8), and endemic human coronavirus (HCoV) spike proteins. SARS-CoV-2 spike-specific cells were enriched in the memory compartment of acutely infected and convalescent patients several months post symptom onset. With severe acute infection, substantial populations of endemic HCoV-reactive antibody-secreting cells were identified and possessed highly mutated variable genes, signifying preexisting immunity. Finally, MBCs exhibited pronounced maturation to NP and ORF8 over time, especially in older patients. Monoclonal antibodies against these targets were non-neutralizing and non-protective in vivo. These findings reveal antibody adaptation to non-neutralizing intracellular antigens during infection, emphasizing the importance of vaccination for inducing neutralizing spike-specific MBCs. |Antibodies, Neutralizing/immunology[MESH] |Antibodies, Viral/*immunology[MESH] |Antibody Formation/genetics/*immunology[MESH] |B-Lymphocytes/*immunology/metabolism[MESH] |COVID-19/*immunology[MESH] |Computational Biology/methods[MESH] |Cross Reactions/immunology[MESH] |Epitope Mapping[MESH] |Female[MESH] |Gene Expression Profiling[MESH] |High-Throughput Nucleotide Sequencing[MESH] |Host-Pathogen Interactions/genetics/*immunology[MESH] |Humans[MESH] |Immunodominant Epitopes/genetics/*immunology[MESH] |Immunologic Memory[MESH] |Male[MESH] |Neutralization Tests[MESH] |SARS-CoV-2/*immunology[MESH] |Single-Cell Analysis/methods[MESH] |Spike Glycoprotein, Coronavirus/immunology[MESH]Profiling B cell immunodominance after SARS-CoV-2 infection reveals an(epmc).pdf DeepDyve Pubget Overpricing