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10.1016/j.bios.2021.113280 http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113280 34020221!8088041!34020221     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 187 (ä): 113280 Nephropedia Template TP {{tp|p=34020221|t=2021. HRPZyme Assisted Recognition of SARS-CoV-2 infection by Optical Measurement (HARIOM) |pdf=|usr=}}{{34020221}} gab.com Text HRPZyme Assisted Recognition of SARS-CoV-2 infection by Optical Measurement (HARIOM) JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=34020221 #FOAvip In order to define public health policies, simple, inexpensive and robust detection methods for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are vital for mass-testing in resource limited settings. The current choice of molecular methods for identification of SARS-CoV-2 infection includes nucleic acid-based testing (NAT) for viral genetic material and antigen-based testing for viral protein identification. Host exposure is detected using antibody detection assays. While NATs require sophisticated instrument and trained manpower, antigen tests are plagued by their low sensitivity and specificity. Thus, a test offering sensitive detection for presence of infection as a colorimetric readout holds promise to enable mass testing in resource constrained environments by minimally trained personnel. Here we present a novel HRPZyme Assisted Recognition of Infection by Optical Measurement (HARIOM) assay which combines specificity of NATs with sensitivity of enzymatic assays resulting in enhanced signal to noise ratios in an easily interpretable colorimetric readout. Using this assay, we could detect up to 10(2) copies of synthetic viral RNA spiked in saliva as a detection matrix. Validating our assay on suspected human subjects, we found concordance with PCR based readouts with visible colorimetric distinction between positive and negative samples in less than an hour. We believe that this assay holds the potential to aid in mass screening to detect SARS-CoV-2 infection by facilitating colorimetric detection with minimal resources and less trained personnel. Twit Text FOAVip HRPZyme Assisted Recognition of SARS-CoV-2 infection by Optical Measurement (HARIOM) ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=34020221 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34020221 #FOAvip English Wikipedia <ref>{{Cite pmid|34020221}}</ref> HRPZyme Assisted Recognition of SARS-CoV-2 infection by Optical Measurement (HARIOM) #MMPMID34020221 Ahmad M; Sharma P; Kamai A; Agrawal A; Faruq M; Kulshreshtha A Biosens Bioelectron 2021[Sep]; 187 (ä): 113280 PMID34020221show ga In order to define public health policies, simple, inexpensive and robust detection methods for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are vital for mass-testing in resource limited settings. The current choice of molecular methods for identification of SARS-CoV-2 infection includes nucleic acid-based testing (NAT) for viral genetic material and antigen-based testing for viral protein identification. Host exposure is detected using antibody detection assays. While NATs require sophisticated instrument and trained manpower, antigen tests are plagued by their low sensitivity and specificity. Thus, a test offering sensitive detection for presence of infection as a colorimetric readout holds promise to enable mass testing in resource constrained environments by minimally trained personnel. Here we present a novel HRPZyme Assisted Recognition of Infection by Optical Measurement (HARIOM) assay which combines specificity of NATs with sensitivity of enzymatic assays resulting in enhanced signal to noise ratios in an easily interpretable colorimetric readout. Using this assay, we could detect up to 10(2) copies of synthetic viral RNA spiked in saliva as a detection matrix. Validating our assay on suspected human subjects, we found concordance with PCR based readouts with visible colorimetric distinction between positive and negative samples in less than an hour. We believe that this assay holds the potential to aid in mass screening to detect SARS-CoV-2 infection by facilitating colorimetric detection with minimal resources and less trained personnel. |*Biosensing Techniques[MESH] |*COVID-19[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques[MESH] |RNA, Viral[MESH] |SARS-CoV-2[MESH] |Saliva[MESH]HRPZyme Assisted Recognition of SARS-CoV-2 infection by Optical Measur(epmc).pdf DeepDyve Pubget Overpricing