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10.15252/embj.2021107786 http://scihub22266oqcxt.onion/10.15252/embj.2021107786 34018203!8209949!34018203     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34018203&cmd=llinks): Failed to open stream: HTTP request failed! 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Molecular basis of cross-species ACE2 interactions with SARS-CoV-2-like viruses of pangolin origin |pdf=|usr=}}{{34018203}} gab.com Text Molecular basis of cross-species ACE2 interactions with SARS-CoV-2-like viruses of pangolin origin JO: EMBO J http://www.kidney.de/pget.php?&tw=1&pmid=34018203 #FOAvip Pangolins have been suggested as potential reservoir of zoonotic viruses, including SARS-CoV-2 causing the global COVID-19 outbreak. Here, we study the binding of two SARS-CoV-2-like viruses isolated from pangolins, GX/P2V/2017 and GD/1/2019, to human angiotensin-converting enzyme 2 (hACE2), the receptor of SARS-CoV-2. We find that the spike protein receptor-binding domain (RBD) of pangolin CoVs binds to hACE2 as efficiently as the SARS-CoV-2 RBD in vitro. Furthermore, incorporation of pangolin CoV RBDs allows entry of pseudotyped VSV particles into hACE2-expressing cells. A screen for binding of pangolin CoV RBDs to ACE2 orthologs from various species suggests a broader host range than that of SARS-CoV-2. Additionally, cryo-EM structures of GX/P2V/2017 and GD/1/2019 RBDs in complex with hACE2 show their molecular binding in modes similar to SARS-CoV-2 RBD. Introducing the Q498H substitution found in pangolin CoVs into the SARS-CoV-2 RBD expands its binding capacity to ACE2 homologs of mouse, rat, and European hedgehog. These findings suggest that these two pangolin CoVs may infect humans, highlighting the necessity of further surveillance of pangolin CoVs. Twit Text FOAVip Molecular basis of cross-species ACE2 interactions with SARS-CoV-2-like viruses of pangolin origin ????EMBO J http://www.kidney.de/pget.php?&tw=1&pmid=34018203 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34018203 #FOAvip English Wikipedia <ref>{{Cite pmid|34018203}}</ref> Molecular basis of cross-species ACE2 interactions with SARS-CoV-2-like viruses of pangolin origin #MMPMID34018203 Niu S; Wang J; Bai B; Wu L; Zheng A; Chen Q; Du P; Han P; Zhang Y; Jia Y; Qiao C; Qi J; Tian WX; Wang HW; Wang Q; Gao GF EMBO J 2021[Aug]; 40 (16): e107786 PMID34018203show ga Pangolins have been suggested as potential reservoir of zoonotic viruses, including SARS-CoV-2 causing the global COVID-19 outbreak. Here, we study the binding of two SARS-CoV-2-like viruses isolated from pangolins, GX/P2V/2017 and GD/1/2019, to human angiotensin-converting enzyme 2 (hACE2), the receptor of SARS-CoV-2. We find that the spike protein receptor-binding domain (RBD) of pangolin CoVs binds to hACE2 as efficiently as the SARS-CoV-2 RBD in vitro. Furthermore, incorporation of pangolin CoV RBDs allows entry of pseudotyped VSV particles into hACE2-expressing cells. A screen for binding of pangolin CoV RBDs to ACE2 orthologs from various species suggests a broader host range than that of SARS-CoV-2. Additionally, cryo-EM structures of GX/P2V/2017 and GD/1/2019 RBDs in complex with hACE2 show their molecular binding in modes similar to SARS-CoV-2 RBD. Introducing the Q498H substitution found in pangolin CoVs into the SARS-CoV-2 RBD expands its binding capacity to ACE2 homologs of mouse, rat, and European hedgehog. These findings suggest that these two pangolin CoVs may infect humans, highlighting the necessity of further surveillance of pangolin CoVs. |Amino Acid Substitution[MESH] |Angiotensin-Converting Enzyme 2/chemistry/*metabolism[MESH] |Animals[MESH] |Betacoronavirus/*physiology[MESH] |Binding Sites[MESH] |HEK293 Cells[MESH] |Hedgehogs/virology[MESH] |Host Specificity[MESH] |Humans[MESH] |Mice[MESH] |Models, Molecular[MESH] |Pangolins/*virology[MESH] |Phylogeny[MESH] |Protein Binding[MESH] |Protein Conformation[MESH] |Rats[MESH] |SARS-CoV-2/*physiology[MESH] |Spike Glycoprotein, Coronavirus/*chemistry/genetics/*metabolism[MESH]Molecular basis of cross-species ACE2 interactions with SARS-CoV-2-lik(epmc).pdf DeepDyve Pubget Overpricing