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10.1371/journal.ppat.1009585 http://scihub22266oqcxt.onion/10.1371/journal.ppat.1009585 34010360!8168874!34010360     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34010360&cmd=llinks): Failed to open stream: HTTP request failed! 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SARS-CoV-2 infection, neuropathogenesis and transmission among deer mice: Implications for spillback to New World rodents |pdf=|usr=}}{{34010360}} gab.com Text SARS-CoV-2 infection, neuropathogenesis and transmission among deer mice: Implications for spillback to New World rodents JO: PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=34010360 #FOAvip Coronavirus disease-19 (COVID-19) emerged in late 2019 in China and rapidly became pandemic. As with other coronaviruses, a preponderance of evidence suggests the virus originated in horseshoe bats (Rhinolophus spp.) and may have infected an intermediate host prior to spillover into humans. A significant concern is that SARS-CoV-2 could become established in secondary reservoir hosts outside of Asia. To assess this potential, we challenged deer mice (Peromyscus maniculatus) with SARS-CoV-2 and found robust virus replication in the upper respiratory tract, lungs and intestines, with detectable viral RNA for up to 21 days in oral swabs and 6 days in lungs. Virus entry into the brain also occurred, likely via gustatory-olfactory-trigeminal pathway with eventual compromise to the blood-brain barrier. Despite this, no conspicuous signs of disease were observed, and no deer mice succumbed to infection. Expression of several innate immune response genes were elevated in the lungs, including IFNalpha, IFNbeta, Cxcl10, Oas2, Tbk1 and Pycard. Elevated CD4 and CD8beta expression in the lungs was concomitant with Tbx21, IFNgamma and IL-21 expression, suggesting a type I inflammatory immune response. Contact transmission occurred from infected to naive deer mice through two passages, showing sustained natural transmission and localization into the olfactory bulb, recapitulating human neuropathology. In the second deer mouse passage, an insertion of 4 amino acids occurred to fixation in the N-terminal domain of the spike protein that is predicted to form a solvent-accessible loop. Subsequent examination of the source virus from BEI Resources determined the mutation was present at very low levels, demonstrating potent purifying selection for the insert during in vivo passage. Collectively, this work has determined that deer mice are a suitable animal model for the study of SARS-CoV-2 respiratory disease and neuropathogenesis, and that they have the potential to serve as secondary reservoir hosts in North America. Twit Text FOAVip SARS-CoV-2 infection, neuropathogenesis and transmission among deer mice: Implications for spillback to New World rodents ????PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=34010360 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34010360 #FOAvip English Wikipedia <ref>{{Cite pmid|34010360}}</ref> SARS-CoV-2 infection, neuropathogenesis and transmission among deer mice: Implications for spillback to New World rodents #MMPMID34010360 Fagre A; Lewis J; Eckley M; Zhan S; Rocha SM; Sexton NR; Burke B; Geiss B; Peersen O; Bass T; Kading R; Rovnak J; Ebel GD; Tjalkens RB; Aboellail T; Schountz T PLoS Pathog 2021[May]; 17 (5): e1009585 PMID34010360show ga Coronavirus disease-19 (COVID-19) emerged in late 2019 in China and rapidly became pandemic. As with other coronaviruses, a preponderance of evidence suggests the virus originated in horseshoe bats (Rhinolophus spp.) and may have infected an intermediate host prior to spillover into humans. A significant concern is that SARS-CoV-2 could become established in secondary reservoir hosts outside of Asia. To assess this potential, we challenged deer mice (Peromyscus maniculatus) with SARS-CoV-2 and found robust virus replication in the upper respiratory tract, lungs and intestines, with detectable viral RNA for up to 21 days in oral swabs and 6 days in lungs. Virus entry into the brain also occurred, likely via gustatory-olfactory-trigeminal pathway with eventual compromise to the blood-brain barrier. Despite this, no conspicuous signs of disease were observed, and no deer mice succumbed to infection. Expression of several innate immune response genes were elevated in the lungs, including IFNalpha, IFNbeta, Cxcl10, Oas2, Tbk1 and Pycard. Elevated CD4 and CD8beta expression in the lungs was concomitant with Tbx21, IFNgamma and IL-21 expression, suggesting a type I inflammatory immune response. Contact transmission occurred from infected to naive deer mice through two passages, showing sustained natural transmission and localization into the olfactory bulb, recapitulating human neuropathology. In the second deer mouse passage, an insertion of 4 amino acids occurred to fixation in the N-terminal domain of the spike protein that is predicted to form a solvent-accessible loop. Subsequent examination of the source virus from BEI Resources determined the mutation was present at very low levels, demonstrating potent purifying selection for the insert during in vivo passage. Collectively, this work has determined that deer mice are a suitable animal model for the study of SARS-CoV-2 respiratory disease and neuropathogenesis, and that they have the potential to serve as secondary reservoir hosts in North America. |Animals[MESH] |Brain/pathology/virology[MESH] |COVID-19/pathology/*physiopathology/*transmission[MESH] |Disease Models, Animal[MESH] |Disease Reservoirs[MESH] |Disease Susceptibility[MESH] |Female[MESH] |Male[MESH] |Peromyscus/*virology[MESH] |Spike Glycoprotein, Coronavirus/genetics[MESH]SARS-CoV-2 infection, neuropathogenesis and transmission among deer mi(epmc).pdf DeepDyve Pubget Overpricing