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10.1038/s41467-021-23185-x http://scihub22266oqcxt.onion/10.1038/s41467-021-23185-x 34006857!8131735!34006857     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 2905 Nephropedia Template TP {{tp|p=34006857|t=2021. A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis |pdf=|usr=}}{{34006857}} gab.com Text A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34006857 #FOAvip The need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/muL and 1.09 copies/muL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities. Twit Text FOAVip A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34006857 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34006857 #FOAvip English Wikipedia <ref>{{Cite pmid|34006857}}</ref> A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis #MMPMID34006857 Xun G; Lane ST; Petrov VA; Pepa BE; Zhao H Nat Commun 2021[May]; 12 (1): 2905 PMID34006857show ga The need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/muL and 1.09 copies/muL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities. |*COVID-19 Nucleic Acid Testing/instrumentation[MESH] |*Point-of-Care Systems[MESH] |COVID-19/*diagnosis[MESH] |Equipment Design[MESH] |Genes, Viral/genetics[MESH] |Humans[MESH] |Limit of Detection[MESH] |Molecular Diagnostic Techniques[MESH] |Nucleic Acid Amplification Techniques[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH] |Saliva/virology[MESH]A rapid, accurate, scalable, and portable testing system for COVID-19 (epmc).pdf DeepDyve Pubget Overpricing