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Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Cell+Rep 2021 ; 35 (8): 109179 Nephropedia Template TP
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Mapping the SARS-CoV-2 spike glycoprotein-derived peptidome presented by HLA class II on dendritic cells #MMPMID34004174
Parker R; Partridge T; Wormald C; Kawahara R; Stalls V; Aggelakopoulou M; Parker J; Powell Doherty R; Ariosa Morejon Y; Lee E; Saunders K; Haynes BF; Acharya P; Thaysen-Andersen M; Borrow P; Ternette N
Cell Rep 2021[May]; 35 (8): 109179 PMID34004174show ga
Understanding and eliciting protective immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an urgent priority. To facilitate these objectives, we profile the repertoire of human leukocyte antigen class II (HLA-II)-bound peptides presented by HLA-DR diverse monocyte-derived dendritic cells pulsed with SARS-CoV-2 spike (S) protein. We identify 209 unique HLA-II-bound peptide sequences, many forming nested sets, which map to sites throughout S including glycosylated regions. Comparison of the glycosylation profile of the S protein to that of the HLA-II-bound S peptides reveals substantial trimming of glycan residues on the latter, likely induced during antigen processing. Our data also highlight the receptor-binding motif in S1 as a HLA-DR-binding peptide-rich region and identify S2-derived peptides with potential for targeting by cross-protective vaccine-elicited responses. Results from this study will aid analysis of CD4(+) T cell responses in infected individuals and vaccine recipients and have application in next-generation vaccine design.
|Amino Acid Sequence[MESH]
|Antigen Presentation[MESH]
|COVID-19/*immunology/virology[MESH]
|Dendritic Cells/*immunology[MESH]
|Epitope Mapping[MESH]
|Epitopes, T-Lymphocyte/immunology[MESH]
|Glycosylation[MESH]
|Histocompatibility Antigens Class II/*immunology[MESH]