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10.1016/j.xpro.2021.100582 http://scihub22266oqcxt.onion/10.1016/j.xpro.2021.100582 34002169!8114807!34002169     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       STAR+Protoc 2021 ; 2 (2): 100582 Nephropedia Template TP {{tp|p=34002169|t=2021. Sample processing and single cell RNA-sequencing of peripheral blood immune cells from COVID-19 patients |pdf=|usr=}}{{34002169}} gab.com Text Sample processing and single cell RNA-sequencing of peripheral blood immune cells from COVID-19 patients JO: STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34002169 #FOAvip Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) allows in-depth assessment of transcriptional changes in immune cells of patients with COVID-19. However, collecting, processing, and analyzing samples from patients with COVID-19 pose many challenges because blood samples may contain infectious virus, identification of immune cell subtypes can be difficult, and biological interpretation of analytical results is complex. To address these issues, we describe a protocol for sample processing, sorting, methanol fixation, and scRNA-seq analysis of PBMCs from frozen buffy coat samples from patients with COVID-19. For complete details on the use and execution of this protocol, please refer to (Yao et al., 2021). Twit Text FOAVip Sample processing and single cell RNA-sequencing of peripheral blood immune cells from COVID-19 patients ????STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34002169 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34002169 #FOAvip English Wikipedia <ref>{{Cite pmid|34002169}}</ref> Sample processing and single cell RNA-sequencing of peripheral blood immune cells from COVID-19 patients #MMPMID34002169 Yao C; Bora SA; Chen P; Goodridge HS; Gharib SA STAR Protoc 2021[Jun]; 2 (2): 100582 PMID34002169show ga Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) allows in-depth assessment of transcriptional changes in immune cells of patients with COVID-19. However, collecting, processing, and analyzing samples from patients with COVID-19 pose many challenges because blood samples may contain infectious virus, identification of immune cell subtypes can be difficult, and biological interpretation of analytical results is complex. To address these issues, we describe a protocol for sample processing, sorting, methanol fixation, and scRNA-seq analysis of PBMCs from frozen buffy coat samples from patients with COVID-19. For complete details on the use and execution of this protocol, please refer to (Yao et al., 2021). |COVID-19/blood/genetics/*immunology/virology[MESH] |Humans[MESH] |Leukocytes, Mononuclear/*immunology[MESH] |RNA, Viral/blood/*genetics[MESH] |SARS-CoV-2/genetics/*immunology[MESH] |Sequence Analysis, RNA/*methods[MESH] |Single-Cell Analysis/*methods[MESH]Sample processing and single cell RNA-sequencing of peripheral blood i(epmc).pdf DeepDyve Pubget Overpricing