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10.4049/immunohorizons.2100011 http://scihub22266oqcxt.onion/10.4049/immunohorizons.2100011 34001652!9190970!34001652     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Immunohorizons 2021 ; 5 (5): 322-335 Nephropedia Template TP {{tp|p=34001652|t=2021. One-Stop Serum Assay Identifies COVID-19 Disease Severity and Vaccination Responses |pdf=|usr=}}{{34001652}} gab.com Text One-Stop Serum Assay Identifies COVID-19 Disease Severity and Vaccination Responses JO: Immunohorizons http://www.kidney.de/pget.php?&tw=1&pmid=34001652 #FOAvip SARS-CoV-2 has caused over 100,000,000 cases and almost 2,500,000 deaths globally. Comprehensive assessment of the multifaceted antiviral Ab response is critical for diagnosis, differentiation of severity, and characterization of long-term immunity, especially as COVID-19 vaccines become available. Severe disease is associated with early, massive plasmablast responses. We developed a multiplex immunoassay from serum/plasma of acutely infected and convalescent COVID-19 patients and prepandemic and postpandemic healthy adults. We measured IgA, IgG, and/or IgM against SARS-CoV-2 nucleocapsid (N), spike domain 1 (S1), S1-receptor binding domain (RBD) and S1-N-terminal domain. For diagnosis, the combined [IgA + IgG + IgM] or IgG levels measured for N, S1, and S1-RBD yielded area under the curve values >/=0.90. Virus-specific Ig levels were higher in patients with severe/critical compared with mild/moderate infections. A strong prozone effect was observed in sera from severe/critical patients-a possible source of underestimated Ab concentrations in previous studies. Mild/moderate patients displayed a slower rise and lower peak in anti-N and anti-S1 IgG levels compared with severe/critical patients, but anti-RBD IgG and neutralization responses reached similar levels at 2-4 mo after symptom onset. Measurement of the Ab responses in sera from 18 COVID-19-vaccinated patients revealed specific responses for the S1-RBD Ag and none against the N protein. This highly sensitive, SARS-CoV-2-specific, multiplex immunoassay measures the magnitude, complexity, and kinetics of the Ab response and can distinguish serum Ab responses from natural SARS-CoV-2 infections (mild or severe) and mRNA COVID-19 vaccines. Twit Text FOAVip One-Stop Serum Assay Identifies COVID-19 Disease Severity and Vaccination Responses ????Immunohorizons http://www.kidney.de/pget.php?&tw=1&pmid=34001652 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34001652 #FOAvip English Wikipedia <ref>{{Cite pmid|34001652}}</ref> One-Stop Serum Assay Identifies COVID-19 Disease Severity and Vaccination Responses #MMPMID34001652 Haddad NS; Nguyen DC; Kuruvilla ME; Morrison-Porter A; Anam F; Cashman KS; Ramonell RP; Kyu S; Saini AS; Cabrera-Mora M; Derrico A; Alter D; Roback JD; Horwath M; O'Keefe JB; Wu HM; Wong AI; Dretler AW; Gripaldo R; Lane AN; Wu H; Chu HY; Lee S; Hernandez M; Engineer V; Varghese J; Patel R; Jalal A; French V; Guysenov I; Lane CE; Mengistsu T; Normile KE; Mnzava O; Le S; Sanz I; Daiss JL; Lee FE Immunohorizons 2021[May]; 5 (5): 322-335 PMID34001652show ga SARS-CoV-2 has caused over 100,000,000 cases and almost 2,500,000 deaths globally. Comprehensive assessment of the multifaceted antiviral Ab response is critical for diagnosis, differentiation of severity, and characterization of long-term immunity, especially as COVID-19 vaccines become available. Severe disease is associated with early, massive plasmablast responses. We developed a multiplex immunoassay from serum/plasma of acutely infected and convalescent COVID-19 patients and prepandemic and postpandemic healthy adults. We measured IgA, IgG, and/or IgM against SARS-CoV-2 nucleocapsid (N), spike domain 1 (S1), S1-receptor binding domain (RBD) and S1-N-terminal domain. For diagnosis, the combined [IgA + IgG + IgM] or IgG levels measured for N, S1, and S1-RBD yielded area under the curve values >/=0.90. Virus-specific Ig levels were higher in patients with severe/critical compared with mild/moderate infections. A strong prozone effect was observed in sera from severe/critical patients-a possible source of underestimated Ab concentrations in previous studies. Mild/moderate patients displayed a slower rise and lower peak in anti-N and anti-S1 IgG levels compared with severe/critical patients, but anti-RBD IgG and neutralization responses reached similar levels at 2-4 mo after symptom onset. Measurement of the Ab responses in sera from 18 COVID-19-vaccinated patients revealed specific responses for the S1-RBD Ag and none against the N protein. This highly sensitive, SARS-CoV-2-specific, multiplex immunoassay measures the magnitude, complexity, and kinetics of the Ab response and can distinguish serum Ab responses from natural SARS-CoV-2 infections (mild or severe) and mRNA COVID-19 vaccines. |*Antibodies, Viral/blood/immunology[MESH] |*COVID-19/blood/immunology/prevention & control[MESH] |*SARS-CoV-2/immunology/metabolism[MESH] |*Severity of Illness Index[MESH] |*Vaccination[MESH] |Adult[MESH] |Aged[MESH] |COVID-19 Vaccines/*administration & dosage[MESH] |Female[MESH] |Humans[MESH] |Immunoassay[MESH] |Male[MESH]One-Stop Serum Assay Identifies COVID-19 Disease Severity and Vaccinat(epmc).pdf DeepDyve Pubget Overpricing