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10.1016/j.matt.2021.05.003

http://scihub22266oqcxt.onion/10.1016/j.matt.2021.05.003
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33997767!8106877!33997767
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suck abstract from ncbi


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pmid33997767      Matter 2021 ; 4 (7): 2403-2416
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  • Low-cost biosensor for rapid detection of SARS-CoV-2 at the point of care #MMPMID33997767
  • Torres MDT; de Araujo WR; de Lima LF; Ferreira AL; de la Fuente-Nunez C
  • Matter 2021[Jul]; 4 (7): 2403-2416 PMID33997767show ga
  • SARS-CoV-2, the virus that causes COVID-19, has killed over 3 million people worldwide. Despite the urgency of the current pandemic, most available diagnostic methods for COVID-19 use RT-PCR to detect nucleic acid sequences specific to SARS-CoV-2. These tests are limited by their requirement of a large laboratory space, high reagent costs, multistep sample preparation, and the potential for cross-contamination. Moreover, results usually take hours to days to become available. Therefore, fast, reliable, inexpensive, and scalable point-of-care diagnostics are urgently needed. Here, we describe RAPID 1.0, a simple, handheld, and highly sensitive miniaturized biosensor modified with human receptor angiotensin-converting enzyme-2. RAPID 1.0 can detect SARS-CoV-2 using 10 muL of sample within 4 min through its increased resistance to charge transfer of a redox probe measured by electrochemical impedance spectroscopy. The sensitivity and specificity of RAPID for nasopharyngeal/oropharyngeal swab and saliva samples are 85.3% and 100% and 100% and 86.5%, respectively.
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