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10.1111/1751-7915.13828 http://scihub22266oqcxt.onion/10.1111/1751-7915.13828 33993637!8209898!33993637     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Microb+Biotechnol 2021 ; 14 (4): 1627-1641 Nephropedia Template TP {{tp|p=33993637|t=2021. SARS-CoV-2 RNA screening in routine pathology specimens |pdf=|usr=}}{{33993637}} gab.com Text SARS-CoV-2 RNA screening in routine pathology specimens JO: Microb Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=33993637 #FOAvip Virus detection methods are important to cope with the SARS-CoV-2 pandemics. Apart from the lung, SARS-CoV-2 was detected in multiple organs in severe cases. Less is known on organ tropism in patients developing mild or no symptoms, and some of such patients might be missed in symptom-indicated swab testing. Here, we tested and validated several approaches and selected the most reliable RT-PCR protocol for the detection of SARS-CoV-2 RNA in patients' routine diagnostic formalin-fixed and paraffin-embedded (FFPE) specimens available in pathology, to assess (i) organ tropism in samples from COVID-19-positive patients, (ii) unrecognized cases in selected tissues from negative or not-tested patients during a pandemic peak, and (iii) retrospectively, pre-pandemic lung samples. We identified SARS-CoV-2 RNA in seven samples from confirmed COVID-19 patients, in two gastric biopsies, one small bowel and one colon resection, one lung biopsy, one pleural resection and one pleural effusion specimen, while all other specimens were negative. In the pandemic peak cohort, we identified one previously unrecognized COVID-19 case in tonsillectomy samples. All pre-pandemic lung samples were negative. In conclusion, SARS-CoV-2 RNA detection in FFPE pathology specimens can potentially improve surveillance of COVID-19, allow retrospective studies, and advance our understanding of SARS-CoV-2 organ tropism and effects. Twit Text FOAVip SARS-CoV-2 RNA screening in routine pathology specimens ????Microb Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=33993637 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33993637 #FOAvip English Wikipedia <ref>{{Cite pmid|33993637}}</ref> SARS-CoV-2 RNA screening in routine pathology specimens #MMPMID33993637 von Stillfried S; Villwock S; Bulow RD; Djudjaj S; Buhl EM; Maurer A; Ortiz-Bruchle N; Celec P; Klinkhammer BM; Wong DWL; Cacchi C; Braunschweig T; Knuchel-Clarke R; Dahl E; Boor P Microb Biotechnol 2021[Jul]; 14 (4): 1627-1641 PMID33993637show ga Virus detection methods are important to cope with the SARS-CoV-2 pandemics. Apart from the lung, SARS-CoV-2 was detected in multiple organs in severe cases. Less is known on organ tropism in patients developing mild or no symptoms, and some of such patients might be missed in symptom-indicated swab testing. Here, we tested and validated several approaches and selected the most reliable RT-PCR protocol for the detection of SARS-CoV-2 RNA in patients' routine diagnostic formalin-fixed and paraffin-embedded (FFPE) specimens available in pathology, to assess (i) organ tropism in samples from COVID-19-positive patients, (ii) unrecognized cases in selected tissues from negative or not-tested patients during a pandemic peak, and (iii) retrospectively, pre-pandemic lung samples. We identified SARS-CoV-2 RNA in seven samples from confirmed COVID-19 patients, in two gastric biopsies, one small bowel and one colon resection, one lung biopsy, one pleural resection and one pleural effusion specimen, while all other specimens were negative. In the pandemic peak cohort, we identified one previously unrecognized COVID-19 case in tonsillectomy samples. All pre-pandemic lung samples were negative. In conclusion, SARS-CoV-2 RNA detection in FFPE pathology specimens can potentially improve surveillance of COVID-19, allow retrospective studies, and advance our understanding of SARS-CoV-2 organ tropism and effects. |*COVID-19/diagnosis[MESH] |*SARS-CoV-2[MESH] |Diagnostic Tests, Routine[MESH] |Humans[MESH] |Pandemics[MESH] |RNA, Viral/*isolation & purification[MESH]SARS-CoV-2 RNA screening in routine pathology specimens (epmc).pdf DeepDyve Pubget Overpricing