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10.1016/j.jviromet.2021.114171 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114171 33984394!ä!33984394 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 294 (ä): 114171 Nephropedia Template TP {{tp|p=33984394|t=2021. Rapid detection of human respiratory syncytial virus A and B by duplex real-time RT-PCR |pdf=|usr=}}{{33984394}} gab.com Text Rapid detection of human respiratory syncytial virus A and B by duplex real-time RT-PCR JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33984394 #FOAvip Respiratory syncytial virus (RSV) is a common cause of acute respiratory disease worldwide, especially in young children. The World Health Organization (WHO) has initiated an RSV Surveillance Pilot program that aims to perform worldwide RSV surveillance, requiring the development of reliable and rapid molecular methods to detect and identify RSV. A duplex real-time RT-PCR assay developed for simultaneous detection of both A and B subtypes of RSV was included as part of this program. This duplex assay targeted a conserved region of the RSV polymerase gene and was validated for analytical sensitivity, specificity, reproducibility and clinical performance with a wide range of respiratory specimens. The assay was highly specific for RSV and did not react with non-RSV respiratory pathogens, including the SARS-CoV-2 virus. Twit Text FOAVip Rapid detection of human respiratory syncytial virus A and B by duplex real-time RT-PCR ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33984394 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33984394 #FOAvip English Wikipedia <ref>{{Cite pmid|33984394}}</ref> Rapid detection of human respiratory syncytial virus A and B by duplex real-time RT-PCR #MMPMID33984394 Todd AK; Costa AM; Waller G; Daley AJ; Barr IG; Deng YM J Virol Methods 2021[Aug]; 294 (ä): 114171 PMID33984394show ga Respiratory syncytial virus (RSV) is a common cause of acute respiratory disease worldwide, especially in young children. The World Health Organization (WHO) has initiated an RSV Surveillance Pilot program that aims to perform worldwide RSV surveillance, requiring the development of reliable and rapid molecular methods to detect and identify RSV. A duplex real-time RT-PCR assay developed for simultaneous detection of both A and B subtypes of RSV was included as part of this program. This duplex assay targeted a conserved region of the RSV polymerase gene and was validated for analytical sensitivity, specificity, reproducibility and clinical performance with a wide range of respiratory specimens. The assay was highly specific for RSV and did not react with non-RSV respiratory pathogens, including the SARS-CoV-2 virus. |DNA Primers/genetics[MESH] |Humans[MESH] |Limit of Detection[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Nasopharynx/virology[MESH] |RNA, Viral/*isolation & purification[MESH] |RNA-Dependent RNA Polymerase/genetics[MESH] |Reproducibility of Results[MESH] |Respiratory Syncytial Virus, Human/*isolation & purification[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH] |Ribonuclease P/genetics[MESH] |SARS-CoV-2/genetics[MESH]Rapid detection of human respiratory syncytial virus A and B by duplex(epmc).pdf DeepDyve Pubget Overpricing