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10.1016/j.jviromet.2021.114183 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114183 33984393!8110331!33984393     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 294 (ä): 114183 Nephropedia Template TP {{tp|p=33984393|t=2021. Development of an immunochromatographic kit to detect severe acute respiratory syndrome coronavirus 2 |pdf=|usr=}}{{33984393}} gab.com Text Development of an immunochromatographic kit to detect severe acute respiratory syndrome coronavirus 2 JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33984393 #FOAvip BACKGROUND: The novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the worldwide coronavirus disease-19 (COVID-19) pandemic, starting in late 2019. The standard diagnostic methods to detect SARS-CoV-2 are PCR-based genetic assays. Antigen-antibody-based immunochromatographic assays are alternative methods of detecting this virus. Rapid diagnosis kits to detect SARS-CoV-2 are urgently needed. STUDY DESIGN: Three monoclonal antibodies against SARS-CoV-2 nucleocapsid (N) protein were used to develop an antigen-antibody-based immunochromatographic kit to detect SARS-CoV-2. These assays were evaluated using? nasopharyngeal swab specimens collected from patients suspected of having COVID-19. RESULTS: These assays detected recombinant SARS-CoV-2?N protein at concentrations >0.2?ng/mL within 10?min after protein loading, but did not detect the N proteins of Middle East respiratory syndrome coronavirus (MERS-CoV), human coronaviruses OC43 (HCoV-OC43) and 299E (HCoV-229E) and other pathogens causing respiratory infections. Nasopharyngeal swab specimens obtained 1?3, 4?9, and >/= 10 days after symptom onset from COVID-19 patients diagnosed by RT-PCR showed positivity rates of 100 %, >80 %, and <30 %, respectively. CONCLUSIONS: Kits using this immunochromatographic assay may be a rapid and useful tool for point-of-care diagnosis of COVID-19 when samples are obtained from patients 1?9 days after symptom onset. Twit Text FOAVip Development of an immunochromatographic kit to detect severe acute respiratory syndrome coronavirus 2 ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33984393 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33984393 #FOAvip English Wikipedia <ref>{{Cite pmid|33984393}}</ref> Development of an immunochromatographic kit to detect severe acute respiratory syndrome coronavirus 2 #MMPMID33984393 Oshiro S; Tabe Y; Funatogawa K; Saito K; Tada T; Hishinuma T; Mizutani N; Akiwa M; Sekiguchi JI; Miida T; Kirikae T J Virol Methods 2021[Aug]; 294 (ä): 114183 PMID33984393show ga BACKGROUND: The novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the worldwide coronavirus disease-19 (COVID-19) pandemic, starting in late 2019. The standard diagnostic methods to detect SARS-CoV-2 are PCR-based genetic assays. Antigen-antibody-based immunochromatographic assays are alternative methods of detecting this virus. Rapid diagnosis kits to detect SARS-CoV-2 are urgently needed. STUDY DESIGN: Three monoclonal antibodies against SARS-CoV-2 nucleocapsid (N) protein were used to develop an antigen-antibody-based immunochromatographic kit to detect SARS-CoV-2. These assays were evaluated using? nasopharyngeal swab specimens collected from patients suspected of having COVID-19. RESULTS: These assays detected recombinant SARS-CoV-2?N protein at concentrations >0.2?ng/mL within 10?min after protein loading, but did not detect the N proteins of Middle East respiratory syndrome coronavirus (MERS-CoV), human coronaviruses OC43 (HCoV-OC43) and 299E (HCoV-229E) and other pathogens causing respiratory infections. Nasopharyngeal swab specimens obtained 1?3, 4?9, and >/= 10 days after symptom onset from COVID-19 patients diagnosed by RT-PCR showed positivity rates of 100 %, >80 %, and <30 %, respectively. CONCLUSIONS: Kits using this immunochromatographic assay may be a rapid and useful tool for point-of-care diagnosis of COVID-19 when samples are obtained from patients 1?9 days after symptom onset. |Animals[MESH] |Antibodies, Monoclonal/immunology[MESH] |COVID-19/blood/*diagnosis[MESH] |Coronavirus Nucleocapsid Proteins/*immunology[MESH] |Humans[MESH] |Immunoassay/*methods[MESH] |Nasopharynx/virology[MESH] |Phosphoproteins/immunology[MESH] |Rats[MESH]Development of an immunochromatographic kit to detect severe acute res(epmc).pdf DeepDyve Pubget Overpricing