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10.1016/j.ebiom.2021.103357 http://scihub22266oqcxt.onion/10.1016/j.ebiom.2021.103357 33979758!8153212!33979758     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       EBioMedicine 2021 ; 67 (ä): 103357 Nephropedia Template TP {{tp|p=33979758|t=2021. Blood neutrophils from children with COVID-19 exhibit both inflammatory and anti-inflammatory markers |pdf=|usr=}}{{33979758}} gab.com Text Blood neutrophils from children with COVID-19 exhibit both inflammatory and anti-inflammatory markers JO: EBioMedicine http://www.kidney.de/pget.php?&tw=1&pmid=33979758 #FOAvip BACKGROUND: Perhaps reflecting that children with COVID-19 rarely exhibit severe respiratory symptoms and often remain asymptomatic, little attention has been paid to explore the immune response in pediatric COVID-19. Here, we analyzed the phenotype and function of circulating neutrophils from children with COVID-19. METHODS: An observational study including 182 children with COVID-19, 21 children with multisystem inflammatory syndrome (MIS-C), and 40 healthy children was performed in Buenos Aires, Argentina. Neutrophil phenotype was analyzed by flow cytometry in blood samples. Cytokine production, plasma levels of IgG antibodies directed to the spike protein of SARS-CoV-2 and citrullinated histone H3 were measured by ELISA. Cell-free DNA was quantified by fluorometry. FINDINGS: Compared with healthy controls, neutrophils from children with COVID-19 showed a lower expression of CD11b, CD66b, and L-selectin but a higher expression of the activation markers HLA-DR, CD64 and PECAM-1 and the inhibitory receptors LAIR-1 and PD-L1. No differences in the production of cytokines and NETs were observed. Interestingly, the expression of CD64 in neutrophils and the serum concentration of IgG antibodies directed to the spike protein of SARS-CoV-2 distinguished asymptomatic from mild and moderate COVID-19. INTERPRETATION: Acute lung injury is a prominent feature of severe COVID-19 in adults. A low expression of adhesion molecules together with a high expression of inhibitory receptors in neutrophils from children with COVID-19 might prevent tissue infiltration by neutrophils preserving lung function. FUNDING: This study was supported by the Ministry of Science and Technology (National Agency for Scientific and Technological Promotion, IP-COVID-19-0277 and PMO BID PICT 2018-2548), and University of Buenos Aires from Argentina (20020170100573BA). Twit Text FOAVip Blood neutrophils from children with COVID-19 exhibit both inflammatory and anti-inflammatory markers ????EBioMedicine http://www.kidney.de/pget.php?&tw=1&pmid=33979758 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33979758 #FOAvip English Wikipedia <ref>{{Cite pmid|33979758}}</ref> Blood neutrophils from children with COVID-19 exhibit both inflammatory and anti-inflammatory markers #MMPMID33979758 Seery V; Raiden SC; Algieri SC; Grisolia NA; Filippo D; De Carli N; Di Lalla S; Cairoli H; Chiolo MJ; Meregalli CN; Gimenez LI; Gregorio G; Sarli M; Alcalde AL; Davenport C; Bruera MJ; Simaz N; Perez MF; Nivela V; Bayle C; Tuccillo P; Agosta MT; Perez H; Villa Nova S; Suarez P; Takata EM; Garcia M; Lattner J; Rolon MJ; Coll P; Sananez I; Holgado MP; Ferrero F; Geffner J; Arruvito L EBioMedicine 2021[May]; 67 (ä): 103357 PMID33979758show ga BACKGROUND: Perhaps reflecting that children with COVID-19 rarely exhibit severe respiratory symptoms and often remain asymptomatic, little attention has been paid to explore the immune response in pediatric COVID-19. Here, we analyzed the phenotype and function of circulating neutrophils from children with COVID-19. METHODS: An observational study including 182 children with COVID-19, 21 children with multisystem inflammatory syndrome (MIS-C), and 40 healthy children was performed in Buenos Aires, Argentina. Neutrophil phenotype was analyzed by flow cytometry in blood samples. Cytokine production, plasma levels of IgG antibodies directed to the spike protein of SARS-CoV-2 and citrullinated histone H3 were measured by ELISA. Cell-free DNA was quantified by fluorometry. FINDINGS: Compared with healthy controls, neutrophils from children with COVID-19 showed a lower expression of CD11b, CD66b, and L-selectin but a higher expression of the activation markers HLA-DR, CD64 and PECAM-1 and the inhibitory receptors LAIR-1 and PD-L1. No differences in the production of cytokines and NETs were observed. Interestingly, the expression of CD64 in neutrophils and the serum concentration of IgG antibodies directed to the spike protein of SARS-CoV-2 distinguished asymptomatic from mild and moderate COVID-19. INTERPRETATION: Acute lung injury is a prominent feature of severe COVID-19 in adults. A low expression of adhesion molecules together with a high expression of inhibitory receptors in neutrophils from children with COVID-19 might prevent tissue infiltration by neutrophils preserving lung function. FUNDING: This study was supported by the Ministry of Science and Technology (National Agency for Scientific and Technological Promotion, IP-COVID-19-0277 and PMO BID PICT 2018-2548), and University of Buenos Aires from Argentina (20020170100573BA). |Antibodies, Viral/blood[MESH] |Argentina[MESH] |Biomarkers/*blood[MESH] |COVID-19/blood/*immunology[MESH] |Case-Control Studies[MESH] |Child[MESH] |Child, Preschool[MESH] |Cytokines/blood[MESH] |Female[MESH] |Flow Cytometry[MESH] |Humans[MESH] |Immunoglobulin G/blood[MESH] |Infant[MESH] |Male[MESH] |Neutrophils/*immunology[MESH] |SARS-CoV-2/immunology[MESH] |Spike Glycoprotein, Coronavirus/immunology[MESH]Blood neutrophils from children with COVID-19 exhibit both inflammator(epmc).pdf DeepDyve Pubget Overpricing