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10.1038/s41467-021-22905-7 http://scihub22266oqcxt.onion/10.1038/s41467-021-22905-7 33976134!8113528!33976134     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 2642 Nephropedia Template TP {{tp|p=33976134|t=2021. SARS-CoV-2 gene content and COVID-19 mutation impact by comparing 44 Sarbecovirus genomes |pdf=|usr=}}{{33976134}} gab.com Text SARS-CoV-2 gene content and COVID-19 mutation impact by comparing 44 Sarbecovirus genomes JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33976134 #FOAvip Despite its clinical importance, the SARS-CoV-2 gene set remains unresolved, hindering dissection of COVID-19 biology. We use comparative genomics to provide a high-confidence protein-coding gene set, characterize evolutionary constraint, and prioritize functional mutations. We select 44 Sarbecovirus genomes at ideally-suited evolutionary distances, and quantify protein-coding evolutionary signatures and overlapping constraint. We find strong protein-coding signatures for ORFs 3a, 6, 7a, 7b, 8, 9b, and a novel alternate-frame gene, ORF3c, whereas ORFs 2b, 3d/3d-2, 3b, 9c, and 10 lack protein-coding signatures or convincing experimental evidence of protein-coding function. Furthermore, we show no other conserved protein-coding genes remain to be discovered. Mutation analysis suggests ORF8 contributes to within-individual fitness but not person-to-person transmission. Cross-strain and within-strain evolutionary pressures agree, except for fewer-than-expected within-strain mutations in nsp3 and S1, and more-than-expected in nucleocapsid, which shows a cluster of mutations in a predicted B-cell epitope, suggesting immune-avoidance selection. Evolutionary histories of residues disrupted by spike-protein substitutions D614G, N501Y, E484K, and K417N/T provide clues about their biology, and we catalog likely-functional co-inherited mutations. Previously reported RNA-modification sites show no enrichment for conservation. Here we report a high-confidence gene set and evolutionary-history annotations providing valuable resources and insights on SARS-CoV-2 biology, mutations, and evolution. Twit Text FOAVip SARS-CoV-2 gene content and COVID-19 mutation impact by comparing 44 Sarbecovirus genomes ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33976134 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33976134 #FOAvip English Wikipedia <ref>{{Cite pmid|33976134}}</ref> SARS-CoV-2 gene content and COVID-19 mutation impact by comparing 44 Sarbecovirus genomes #MMPMID33976134 Jungreis I; Sealfon R; Kellis M Nat Commun 2021[May]; 12 (1): 2642 PMID33976134show ga Despite its clinical importance, the SARS-CoV-2 gene set remains unresolved, hindering dissection of COVID-19 biology. We use comparative genomics to provide a high-confidence protein-coding gene set, characterize evolutionary constraint, and prioritize functional mutations. We select 44 Sarbecovirus genomes at ideally-suited evolutionary distances, and quantify protein-coding evolutionary signatures and overlapping constraint. We find strong protein-coding signatures for ORFs 3a, 6, 7a, 7b, 8, 9b, and a novel alternate-frame gene, ORF3c, whereas ORFs 2b, 3d/3d-2, 3b, 9c, and 10 lack protein-coding signatures or convincing experimental evidence of protein-coding function. Furthermore, we show no other conserved protein-coding genes remain to be discovered. Mutation analysis suggests ORF8 contributes to within-individual fitness but not person-to-person transmission. Cross-strain and within-strain evolutionary pressures agree, except for fewer-than-expected within-strain mutations in nsp3 and S1, and more-than-expected in nucleocapsid, which shows a cluster of mutations in a predicted B-cell epitope, suggesting immune-avoidance selection. Evolutionary histories of residues disrupted by spike-protein substitutions D614G, N501Y, E484K, and K417N/T provide clues about their biology, and we catalog likely-functional co-inherited mutations. Previously reported RNA-modification sites show no enrichment for conservation. Here we report a high-confidence gene set and evolutionary-history annotations providing valuable resources and insights on SARS-CoV-2 biology, mutations, and evolution. |*Mutation[MESH] |Betacoronavirus/classification/genetics[MESH] |COVID-19/*virology[MESH] |Codon[MESH] |Evolution, Molecular[MESH] |Genes, Viral[MESH] |Genetic Fitness[MESH] |Genetic Variation[MESH] |Genome, Viral/*genetics[MESH] |Open Reading Frames[MESH] |Phylogeny[MESH] |SARS-CoV-2/*genetics[MESH] |Spike Glycoprotein, Coronavirus/genetics[MESH]SARS-CoV-2 gene content and COVID-19 mutation impact by comparing 44 S(epmc).pdf DeepDyve Pubget Overpricing