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10.1016/j.meegid.2021.104910 http://scihub22266oqcxt.onion/10.1016/j.meegid.2021.104910 33975021!8105304!33975021     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Infect+Genet+Evol 2021 ; 92 (ä): 104910 Nephropedia Template TP {{tp|p=33975021|t=2021. A Sanger-based approach for scaling up screening of SARS-CoV-2 variants of interest and concern |pdf=|usr=}}{{33975021}} gab.com Text A Sanger-based approach for scaling up screening of SARS-CoV-2 variants of interest and concern JO: Infect Genet Evol http://www.kidney.de/pget.php?&tw=1&pmid=33975021 #FOAvip The global spread of new SARS-CoV-2 variants of concern underscore an urgent need of simple deployed molecular tools that can differentiate these lineages. Several tools and protocols have been shared since the beginning of the COVID-19 pandemic, but they need to be timely adapted to cope with SARS-CoV-2 evolution. Although whole-genome sequencing (WGS) of the virus genetic material has been widely used, it still presents practical difficulties such as high cost, shortage of available reagents in the global market, need of a specialized laboratorial infrastructure and well-trained staff. These limitations result in SARS-CoV-2 surveillance blackouts across several countries. Here we propose a rapid and accessible protocol based on Sanger sequencing of a single PCR fragment that is able to identify and discriminate all SARS-CoV-2 variants of concern (VOCs) identified so far, according to each characteristic mutational profile at the Spike-RBD region (K417N/T, E484K, N501Y, A570D). Twelve COVID-19 samples from Brazilian patients were evaluated for both WGS and Sanger sequencing: three P.2, two P.1, six B.1.1 and one B.1.1.117 lineage. All results from the Sanger sequencing method perfectly matched the mutational profile of VOCs and non-VOCs RBD's characterized by WGS. In summary, this approach allows a much broader network of laboratories to perform molecular surveillance of SARS-CoV-2 VOCs and report results within a shorter time frame, which is of utmost importance in the context of rapid public health decisions in a fast evolving worldwide pandemic. Twit Text FOAVip A Sanger-based approach for scaling up screening of SARS-CoV-2 variants of interest and concern ????Infect Genet Evol http://www.kidney.de/pget.php?&tw=1&pmid=33975021 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33975021 #FOAvip English Wikipedia <ref>{{Cite pmid|33975021}}</ref> A Sanger-based approach for scaling up screening of SARS-CoV-2 variants of interest and concern #MMPMID33975021 Bezerra MF; Machado LC; De Carvalho VDCV; Docena C; Brandao-Filho SP; Ayres CFJ; Paiva MHS; Wallau GL Infect Genet Evol 2021[Aug]; 92 (ä): 104910 PMID33975021show ga The global spread of new SARS-CoV-2 variants of concern underscore an urgent need of simple deployed molecular tools that can differentiate these lineages. Several tools and protocols have been shared since the beginning of the COVID-19 pandemic, but they need to be timely adapted to cope with SARS-CoV-2 evolution. Although whole-genome sequencing (WGS) of the virus genetic material has been widely used, it still presents practical difficulties such as high cost, shortage of available reagents in the global market, need of a specialized laboratorial infrastructure and well-trained staff. These limitations result in SARS-CoV-2 surveillance blackouts across several countries. Here we propose a rapid and accessible protocol based on Sanger sequencing of a single PCR fragment that is able to identify and discriminate all SARS-CoV-2 variants of concern (VOCs) identified so far, according to each characteristic mutational profile at the Spike-RBD region (K417N/T, E484K, N501Y, A570D). Twelve COVID-19 samples from Brazilian patients were evaluated for both WGS and Sanger sequencing: three P.2, two P.1, six B.1.1 and one B.1.1.117 lineage. All results from the Sanger sequencing method perfectly matched the mutational profile of VOCs and non-VOCs RBD's characterized by WGS. In summary, this approach allows a much broader network of laboratories to perform molecular surveillance of SARS-CoV-2 VOCs and report results within a shorter time frame, which is of utmost importance in the context of rapid public health decisions in a fast evolving worldwide pandemic. |*Genetic Variation[MESH] |COVID-19/*virology[MESH] |Gene Expression Regulation, Viral[MESH] |Humans[MESH] |Reproducibility of Results[MESH] |SARS-CoV-2/*genetics[MESH]A Sanger-based approach for scaling up screening of SARS-CoV-2 variant(epmc).pdf DeepDyve Pubget Overpricing