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10.1073/pnas.2100170118 http://scihub22266oqcxt.onion/10.1073/pnas.2100170118 33972410!8166198!33972410     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2021 ; 118 (21): ä Nephropedia Template TP {{tp|p=33972410|t=2021. 2 -O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography |pdf=|usr=}}{{33972410}} gab.com Text 2 -O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=33972410 #FOAvip The genome of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coronavirus has a capping modification at the 5'-untranslated region (UTR) to prevent its degradation by host nucleases. These modifications are performed by the Nsp10/14 and Nsp10/16 heterodimers using S-adenosylmethionine as the methyl donor. Nsp10/16 heterodimer is responsible for the methylation at the ribose 2'-O position of the first nucleotide. To investigate the conformational changes of the complex during 2'-O methyltransferase activity, we used a fixed-target serial synchrotron crystallography method at room temperature. We determined crystal structures of Nsp10/16 with substrates and products that revealed the states before and after methylation, occurring within the crystals during the experiments. Here we report the crystal structure of Nsp10/16 in complex with Cap-1 analog ((m7)GpppA(m2'-O)). Inhibition of Nsp16 activity may reduce viral proliferation, making this protein an attractive drug target. Twit Text FOAVip 2 -O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=33972410 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33972410 #FOAvip English Wikipedia <ref>{{Cite pmid|33972410}}</ref> 2 -O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography #MMPMID33972410 Wilamowski M; Sherrell DA; Minasov G; Kim Y; Shuvalova L; Lavens A; Chard R; Maltseva N; Jedrzejczak R; Rosas-Lemus M; Saint N; Foster IT; Michalska K; Satchell KJF; Joachimiak A Proc Natl Acad Sci U S A 2021[May]; 118 (21): ä PMID33972410show ga The genome of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coronavirus has a capping modification at the 5'-untranslated region (UTR) to prevent its degradation by host nucleases. These modifications are performed by the Nsp10/14 and Nsp10/16 heterodimers using S-adenosylmethionine as the methyl donor. Nsp10/16 heterodimer is responsible for the methylation at the ribose 2'-O position of the first nucleotide. To investigate the conformational changes of the complex during 2'-O methyltransferase activity, we used a fixed-target serial synchrotron crystallography method at room temperature. We determined crystal structures of Nsp10/16 with substrates and products that revealed the states before and after methylation, occurring within the crystals during the experiments. Here we report the crystal structure of Nsp10/16 in complex with Cap-1 analog ((m7)GpppA(m2'-O)). Inhibition of Nsp16 activity may reduce viral proliferation, making this protein an attractive drug target. |Crystallography[MESH] |Methylation[MESH] |Methyltransferases/chemistry/metabolism[MESH] |Multiprotein Complexes/chemistry/metabolism[MESH] |RNA Cap Analogs/chemistry/metabolism[MESH] |RNA Caps/chemistry/*metabolism[MESH] |RNA, Messenger/chemistry/*metabolism[MESH] |RNA, Viral/chemistry/*metabolism[MESH] |S-Adenosylhomocysteine/chemistry/metabolism[MESH] |S-Adenosylmethionine/chemistry/metabolism[MESH] |SARS-CoV-2/*chemistry/genetics/metabolism[MESH] |Synchrotrons[MESH] |Viral Nonstructural Proteins/chemistry/metabolism[MESH]2 -O methylation of RNA cap in SARS-CoV-2 captured by serial crystallo(epmc).pdf DeepDyve Pubget Overpricing