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10.1016/j.jcv.2021.104846 http://scihub22266oqcxt.onion/10.1016/j.jcv.2021.104846 33971580!8105081!33971580     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2021 ; 140 (ä): 104846 Nephropedia Template TP {{tp|p=33971580|t=2021. SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus |pdf=|usr=}}{{33971580}} gab.com Text SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=33971580 #FOAvip BACKGROUND: The COVID-19 pandemic has highlighted the need for rapid, cost effective and easy-to-use diagnostic tools for SARS-CoV-2 infections that can be used in point of care settings to limit disease transmission. OBJECTIVE: We evaluated two rapid antigen immunochromatographic tests, Abbott Panbio COVID-19 Ag Rapid Test (Panbio) and Zhejiang Orient Gene/Healgen Biotech Coronavirus Ag rapid test cassette (Orient gene) for detection of infectious SARS-CoV-2. RESULTS: The tests were evaluated on nasopharyngeal samples taken from individuals having respiratory and/or COVID-19 related symptoms, which had been analyzed for SARS-CoV-2 RNA using real-time PCR. In total 156 PCR-positive, and 130 (Panbio) and 176 (Orient Gene) PCR-negative samples were analyzed. Overall sensitivity and specificity were 71.8% and 100% for Panbio and 79.5% and 74.4% for the Orient Gene test respectively. The false positives by the Orient Gene test were verified as SARS-CoV-2 negative by in-house real-time PCR assay and were negative for the four seasonal coronaviruses. Subgroup analysis revealed that the antigen tests had high sensitivity for samples with Ct-values <25 (>88%) and for samples containing infectious viruses as determined by cultivation on Vero cells, 94.1% and 97.1% for the Panbio and Orient gene tests, respectively. Furthermore, both tests had a sensitivity of <50 picogram for nucleocapsid protein. No sample with a Ct-value >27 was shown to contain infectious virus. CONCLUSION: The results indicate that the rapid antigen tests, especially the Panbio tests may be a valuable tool to detect contagious persons during the ongoing pandemic. Twit Text FOAVip SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=33971580 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33971580 #FOAvip English Wikipedia <ref>{{Cite pmid|33971580}}</ref> SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus #MMPMID33971580 Nordgren J; Sharma S; Olsson H; Jamtberg M; Falkeborn T; Svensson L; Hagbom M J Clin Virol 2021[Jul]; 140 (ä): 104846 PMID33971580show ga BACKGROUND: The COVID-19 pandemic has highlighted the need for rapid, cost effective and easy-to-use diagnostic tools for SARS-CoV-2 infections that can be used in point of care settings to limit disease transmission. OBJECTIVE: We evaluated two rapid antigen immunochromatographic tests, Abbott Panbio COVID-19 Ag Rapid Test (Panbio) and Zhejiang Orient Gene/Healgen Biotech Coronavirus Ag rapid test cassette (Orient gene) for detection of infectious SARS-CoV-2. RESULTS: The tests were evaluated on nasopharyngeal samples taken from individuals having respiratory and/or COVID-19 related symptoms, which had been analyzed for SARS-CoV-2 RNA using real-time PCR. In total 156 PCR-positive, and 130 (Panbio) and 176 (Orient Gene) PCR-negative samples were analyzed. Overall sensitivity and specificity were 71.8% and 100% for Panbio and 79.5% and 74.4% for the Orient Gene test respectively. The false positives by the Orient Gene test were verified as SARS-CoV-2 negative by in-house real-time PCR assay and were negative for the four seasonal coronaviruses. Subgroup analysis revealed that the antigen tests had high sensitivity for samples with Ct-values <25 (>88%) and for samples containing infectious viruses as determined by cultivation on Vero cells, 94.1% and 97.1% for the Panbio and Orient gene tests, respectively. Furthermore, both tests had a sensitivity of <50 picogram for nucleocapsid protein. No sample with a Ct-value >27 was shown to contain infectious virus. CONCLUSION: The results indicate that the rapid antigen tests, especially the Panbio tests may be a valuable tool to detect contagious persons during the ongoing pandemic. |*COVID-19/diagnosis[MESH] |*SARS-CoV-2/isolation & purification[MESH] |Animals[MESH] |Antigens, Viral/*analysis[MESH] |COVID-19 Testing[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH] |Pandemics[MESH] |RNA, Viral[MESH] |Sensitivity and Specificity[MESH]SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious v(epmc).pdf DeepDyve Pubget Overpricing